Intra-axonal Synthesis of SNAP25 Is Required for the Formation of Presynaptic Terminals.
Intra-axonal Synthesis of SNAP25 Is Required for the Formation of Presynaptic Terminals.
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DOI：
10.1016/j.celrep.2017.08.097
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发表时间：
2017-09-26
期刊：
影响因子：
8.8
通讯作者：
Hengst U
中科院分区：
文献类型：
--
作者：
Batista AFR;Martínez JC;Hengst U
Localized protein synthesis is a mechanism for developing axons to react acutely and in a spatially restricted manner to extracellular signals. As such it is important for many aspects of axonal development, but its role in the formation of presynapses remains poorly understood. We found that the induced assembly of presynaptic terminals required local protein synthesis. Newly synthesized proteins were detectable at nascent presynapses within 15 minutes of inducing synapse formation in isolated axons. The transcript for the t-SNARE protein SNAP25, which is required for the fusion of synaptic vesicles with the plasma membrane, was recruited to presynaptic sites and locally translated. Inhibition of intra-axonal SNAP25 synthesis affected the clustering of SNAP25 and other presynaptic proteins and interfered with the release of synaptic vesicles from presynaptic sites. This study reveals a critical role for the axonal synthesis of SNAP25 in the assembly of presynaptic terminals. Batista et al. find that during the assembly of presynaptic terminals, mRNA translation is upregulated at the nascent presynapses and required for the clustering of presynaptic proteins. Inhibition of local SNAP25 synthesis prevents proper formation of presynaptic terminal and interferes with synaptic vesicle release.
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DOI：
10.1523/jneurosci.2944-12.2013
发表时间：
2013-03-27
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Taylor AM;Wu J;Tai HC;Schuman EM
通讯作者：
Schuman EM
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DOI：
10.1523/jneurosci.6130-08.2009
发表时间：
2009-04-15
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
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通讯作者：
Cotman CW
影响因子：
11
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
21.3
作者：
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通讯作者：
Jaffrey, Samie R.