Abnormal expression of seven myogenesis-related genes in extraocular muscles of patients with concomitant strabismus.
Abnormal expression of seven myogenesis-related genes in extraocular muscles of patients with concomitant strabismus.
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伴有斜视的患者眼外肌中七个肌生成相关基因的异常表达。
DOI：
10.3892/mmr.2012.1149
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发表时间：
2013
影响因子：
3.4
通讯作者：
HUANGXUAN SHEN
中科院分区：
文献类型：
--
作者：
CHONGDE LONG;GUORONG JIN;QINGJIONG ZHANG;HUANGXUAN SHEN
肌肉的增生或发育不全逐渐导致斜视。肌生成相关基因参与眼外肌发育，包括肌生成分化1（MYOD 1）、肌生成素（MYOG）、视网膜母细胞瘤1（RB 1）、细胞周期蛋白依赖性激酶抑制剂1A（P21）、细胞周期蛋白依赖性激酶抑制剂1C（P57）、胰岛素样生长因子1（IGF 1）和肌肉肌酸激酶（MCK）。本研究通过实时定量RT-PCR评估了上述7个肌发生相关基因在18例共同性斜视患者的眼外肌和12例正常对照肌肉样本中的表达，其中12例正常对照肌肉样本来自一名假定健康的男性突然死亡后6 h。我们发现，尽管6个成肌相关调控因子的表达水平之间存在很大差异，但在所有正常肌肉（包括协同肌、拮抗肌和轭肌）中的相对表达模式是相似的。然而，他们的表达水平在18个患病的眼外肌是异常的，所有的基因的表达水平，除了P57，在大多数患病的肌肉组织减少。这些结果表明，这些肌细胞发生相关基因的异常表达可能有助于共同性斜视。
Hyperplasia or hypoplasia of muscles gradually leads to strabismus. Myogenesis-related genes are involved in extraocular muscle development, including myogenic differentiation 1 (MYOD1), myogenin (MYOG), retinoblastoma 1 (RB1), cyclin-dependent kinase inhibitor 1A (P21), cyclin‑dependent kinase inhibitor 1C (P57), insulin-like growth factor 1 (IGF1) and muscle creatine kinase (MCK). This study evaluated the expression of the above seven myogenesis-related genes by real-time quantitative RT-PCR in 18 resected extrocular muscles of patients with concomitant strabismus and 12 normal control muscle samples from one presumably healthy male 6 h after sudden mortality. We found that although there was a great divergence among the expression levels of 6 myogenesis-related regulatory factors, the relative expression patterns were similar in all the normal muscles, including the synergistic, antagonistic and yoke muscles. However, their expression levels in the 18 diseased extraocular muscles were abnormal; the expression levels of all the genes, with the exception of P57, were reduced in most of the diseased muscle tissues. These results imply that the abnormal expression of these myogenesis-related genes may contribute to concomitant strabismus.
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影响因子：
1.8
作者：
Wilmer, Jeremy B.;Backus, Benjamin T.
通讯作者：
Backus, Benjamin T.
DOI：
10.3368/aoj.51.1.67
发表时间：
2001-01
期刊：
American Orthoptic Journal
影响因子：
--
作者：
E. Traboulsi
通讯作者：
E. Traboulsi
DOI：
10.1067/mpa.2002.123656
发表时间：
2002-08
期刊：
Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus
影响因子：
--
作者：
Mei Mellott;W. Scott;G. Ganser;R. Keech
通讯作者：
Mei Mellott;W. Scott;G. Ganser;R. Keech
影响因子：
9.2
作者：
Novitch, BG;Spicer, DB;Lassar, AB
通讯作者：
Lassar, AB
影响因子：
2.7
作者：
Cornelison, DDW;Olwin, BB;Wold, BJ
通讯作者：
Wold, BJ