Separation of basic proteins by capillary electrophoresis using cross-linked polyacrylamide-coated capillaries and cationic buffer additives
Separation of basic proteins by capillary electrophoresis using cross-linked polyacrylamide-coated capillaries and cationic buffer additives
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使用交联聚丙烯酰胺包被的毛细管和阳离子缓冲添加剂通过毛细管电泳分离碱性蛋白质
DOI：
10.1016/0021-9673(93)87011-a
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发表时间：
1993
影响因子：
4.1
通讯作者：
J. Díez
中科院分区：
文献类型：
--
作者：
A. Cifuentes;M. Frutos;J. M. Santos;J. Díez
本文描述了一种在内壁上涂敷交联聚丙烯酰胺涂层的熔融石英毛细管的制备方法。当这些毛细管与含有0.25M吗啉作为阳离子添加剂的酸性分离缓冲液一起使用时，对碱性蛋白质（pI 7 -11）的分离效率可达3 ·105 ~ 5 ·105板/m。这些毛细管的迁移时间的再现性优于1%的运行到运行和2%的毛细管到毛细管。毛细管可在pH 2-10范围内使用90 h以上，而对测定的碱性蛋白的迁移时间或效率没有任何实质性改变。它还表明，毛细管可以在空气中储存超过90天，没有明显的变化，在迁移时间或几种碱性蛋白质的效率。
A method for the preparation of fused-silica capillaries with a cross-linked polyacrylamide coating bonded to the internal wall is described. When these capillaries are used with acidic separation buffers containing 0.25Mmorpholine as cationic additive to mask the residual effect of the negative charges on the silica surface, efficiencies between 3 · 105and 5 · 105plates/m can be obtained for basic proteins (pI7–11). The reproducibility of migration times for these capillaries is better than 1% run-to-run and 2% capillary-to-capillary. The capillaries can be used for more than 90 h over the pH range 2–10 without any substantial alteration of the migration time or efficiency for the basic proteins assayed. It is also shown that the capillaries can be stored for more than 90 days in air with no appreciable variation in the migration time or the efficiency for several basic proteins.
影响因子：
7.4
作者：
COBB, KA;DOLNIK, V;NOVOTNY, M
通讯作者：
NOVOTNY, M
DOI：
10.1016/s0021-9673(01)90205-5
发表时间：
1990-09
期刊：
Journal of chromatography
影响因子：
--
作者：
J. Towns;F. Regnier
通讯作者：
J. Towns;F. Regnier