NO-producing compounds transform neuron responses to glutamate
NO-producing compounds transform neuron responses to glutamate
复制标题
产生 NO 的化合物改变神经元对谷氨酸的反应
DOI：
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复制
发表时间：
2000
影响因子：
--
通讯作者：
T. D’yakonova
中科院分区：
文献类型：
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作者：
T. D’yakonova
我们之前已经证明，一氧化氮增加谷氨酸的兴奋作用，并阻止普通蜗牛大脑中神经元对谷氨酸的脱敏。本研究的目的是确定一氧化氮对这种软体动物神经元对谷氨酸的抑制反应的可能影响。电生理调查进行了三个确定的神经元。结果表明，谷氨酸（0.05 ~ 0.1 mM）初始诱导超极化并阻断这些神经元的尖峰活动。同时暴露于谷氨酸和一氧化氮供体硝普赛或与一氧化氮供体预孵育的效果是细胞再次对谷氨酸产生去极化和兴奋反应。这种转化的兴奋反应持续了几分钟，即使在洗涤24小时后也能重现。一氧化氮合酶阻滞剂单甲基精氨酸阻断了对谷氨酸的兴奋反应。另一种谷氨酸受体激动剂n -甲基- d -天冬氨酸（NMDA, 0.1-1 mM）最初对这些神经元有兴奋作用；在一氧化氮供体对谷氨酸的反应发生转变后，这种效果显著增强。本研究结果表明，NO参与了将谷氨酸抑制反应转化为兴奋反应的过程，这种作用可能是由nmda型受体介导的。
We have previously shown that NO increases the excitatory effects of glutamate and blocks the desensitization of neurons to glutamate in the brain of the common snail. The aim of the present work was to identify the possible effect of NO on inhibitory responses to glutamate in the neurons of this mollusk. Electrophysiological investigations were performed on three identified neurons. The results showed that glutamate (0.05–0.1 mM) initially induced hyperpolarization and blocked the spike activity of these neurons. Simultaneous exposure to glutamate and the NO donor nitroprusside or preincubation with an NO donor had the effect that cells again responded to glutamate with depolarization and excitation. The transformed excitatory response lasted several minutes and could be reproduced even after 24 h of washing. The NO synthase blocker monomethylarginine blocked the excitatory response to glutamate. Another agonist of glutamate receptors, N-methyl-D-aspartate (NMDA, 0.1–1 mM), initially had excitatory effects on these neurons; this effect was significantly enhanced after transformation of the response to glutamate by NO donors. The results obtained here show that NO is involved in transforming the inhibitory responses to glutamate to excitatory responses, and that this effect may be mediated by NMDA-type receptors.
DOI：
10.1073/pnas.88.24.11285
发表时间：
1991-12-01
影响因子：
11.1
作者：
ODELL, TJ;HAWKINS, RD;ARANCIO, O
通讯作者：
ARANCIO, O
Quisqualate 和 ACPD 是已鉴定的海兔神经元中谷氨酸激活电流的激动剂。
影响因子：
2.5
作者：
Katz,PS;Levitan,IB
通讯作者：
Levitan,IB