Intracellular chloride activities in canine tracheal epithelium. Direct evidence for sodium-coupled intracellular chloride accumulation in a chloride-secreting epithelium.
Intracellular chloride activities in canine tracheal epithelium. Direct evidence for sodium-coupled intracellular chloride accumulation in a chloride-secreting epithelium.
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犬气管上皮细胞内氯化物活性。
DOI：
--
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发表时间：
1983
影响因子：
15.9
通讯作者：
M. Welsh
中科院分区：
文献类型：
--
作者：
M. Welsh
犬气管上皮分泌氯通过产电运输过程，似乎适用于各种各样的分泌上皮。为了研究所涉及的机制，细胞内氯离子活性，acCl，测定与Cl-选择性细胞内微电极。结果表明，当分泌速率最低时，acCl为37 mM，刺激分泌时，细胞内电压去极化，但acCl在39 mM时没有明显改变。这些结果表明：（a）在非分泌和分泌条件下，Cl在基底外侧膜上的累积活性分别为平衡分布的3.8和2.4倍;（B）在22 mV的电化学驱动力下，Cl穿过顶膜的出口可能是被动的;（c）刺激分泌增强了Cl穿过基底外侧膜的进入速率，因为Cl转运增加而acCl没有变化。在细胞外液中不存在Na的情况下，acCl接近平衡分布的预期值。这一发现表明，“上坡”进入细胞的Cl对它的电化学梯度是依赖于，并通电，进入Na的梯度。粘膜下布美他尼（一种袢利尿剂）也降低了Cl分泌速率，并降低了acCl，表明Cl进入抑制。这些研究结果表明，Cl进入细胞是针对其电化学梯度，并介导的Na-耦合，布美他尼，bumetanide-refractitable，运输过程中在基底外侧膜和Cl可能退出被动下降一个有利的电化学梯度跨顶膜。
Canine tracheal epithelium secretes Cl via an electrogenic transport process that appears to apply to a wide variety of secretory epithelia. To examine the mechanisms involved, intracellular chloride activity, acCl, was measured with Cl-selective intracellular microelectrodes. The results indicate that when the rate of secretion was minimal acCl was 37 mM; with stimulation of secretion the intracellular voltage depolarized, but acCl was not significantly altered, at 39 mM. These findings indicate that: (a) Cl is accumulated across the basolateral membrane under nonsecreting and secreting conditions at an activity 3.8 and 2.4 times, respectively, that predicted for an equilibrium distribution; (b) Cl exit across the apical membrane may be passive with an electrochemical driving force of 22 mV; and (c) stimulation of secretion enhanced the rate of Cl entry across the basolateral membrane, since Cl transport increased without a change in acCl. In the absence of Na in the extracellular fluid, acCl approached the value expected for an equilibrium distribution. This finding suggests that "uphill" entry of Cl into the cell against its electrochemical gradient is dependent upon, and energized by, the entry of Na down its gradient. Submucosal bumetanide, a loop diuretic, also decreased the rate of Cl secretion and decreased acCl, indicating an inhibition of Cl entry. These findings indicate that Cl entry into the cell is directed against its electrochemical gradient and is mediated by a Na-coupled, bumetanide-inhibitable, transport process at the basolateral membrane and that Cl may exit passively down a favorable electrochemical gradient across the apical membrane.
离体灌注鲨鱼直肠腺中的细胞内氯化物活动。
DOI：
10.1152/ajprenal.1983.245.5.f640
发表时间：
1983
期刊：
The American journal of physiology
影响因子：
--
作者：
Welsh,MJ;Smith,PL;Frizzell,RA
通讯作者：
Frizzell,RA
“袢”利尿剂对狗气管上皮离子转运的影响。
DOI：
10.1152/ajpcell.1983.245.5.c388
发表时间：
1983
期刊：
The American journal of physiology
影响因子：
--
作者：
Widdicombe,JH;Nathanson,IT;Highland,E
通讯作者：
Highland,E
狗气管上皮分离细胞的离子含量和其他特性。
DOI：
10.1152/ajpcell.1981.241.5.c184
发表时间：
1981
期刊：
The American journal of physiology
影响因子：
--
作者：
Widdicombe,JH;Basbaum,CB;Highland,E
通讯作者：
Highland,E