Improving the clinical outcomes by extended culture of day 3 embryos with low blastomere number to blastocyst stage following frozen-thawed embryo transfer.
Improving the clinical outcomes by extended culture of day 3 embryos with low blastomere number to blastocyst stage following frozen-thawed embryo transfer.
复制标题
通过冻结透射式胚胎转移后，第3天的胚胎数量低的胚胎数低的胚胎数量的扩展培养物改善了临床结果。
DOI：
10.1007/s00404-020-05774-1
复制
发表时间：
2021-03
影响因子：
2.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Li B;Huang J;Li L;He X;Wang M;Zhang H;He Y;Kang B;Shi Y;Chen S;Wang X
本研究旨在探讨冻融胚胎移植后低卵裂球数的第3天（D3）胚胎延长培养至囊胚是否改善了临床结局。回顾性分析唐都医院行体外受精/卵胞浆内单精子注射（IVF/ICSI）周期的妇女的临床资料。根据D3期胚胎卵裂球数将患者分为4-5、6、7-9和> 9个细胞组。比较两组的临床疗效。在新鲜移植周期中，4-5和6个细胞组的着床率和临床妊娠率显著低于7-9和> 9个细胞组，而流产率显著高于7-9和> 9个细胞组。在冻融移植周期中，4-5和6个细胞组与7-9和> 9个细胞组相比，单个囊胚移植周期的临床妊娠率和着床率无显著性差异。但4-5个细胞组的流产率明显高于7-9个细胞组和> 9个细胞组。在双胚泡移植周期中，4-5、6、7-9细胞组的临床妊娠率无显著差异。D3期6细胞胚胎的着床率和临床妊娠率明显下降，这些胚胎不被认为是优质胚胎。将具有≤ 6个卵裂球的D3胚胎延长培养至囊胚，特别是6细胞胚胎，导致与来自具有≥ 7个卵裂球的D3胚胎的囊胚相似的临床妊娠率。
This study aimed to investigate whether the extended culture of day 3 (D3) embryos with low blastomere number to blastocyst following frozen–thawed embryo transfer improved the clinical outcomes. This was a retrospective study of clinical data of women undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles in the Tangdu Hospital. The patients were divided into groups with 4–5, 6, 7–9 and > 9 cells based on the blastomere number of D3 embryos. The clinical outcomes were compared. In fresh transfer cycles, the implantation and clinical pregnancy rates significantly decreased, while the abortion rate significantly increased in the groups with 4–5 and 6 cells compared with those with 7–9 and > 9 cells. In frozen–thawed transfer cycles, the clinical pregnancy and implantation rates for a single blastocyst transfer cycle showed no significant differences in the groups with 4–5 and 6 cells compared with those with 7–9 and > 9 cells. However, the abortion rate was significantly higher in the group with 4–5 cells than in that with 7–9 and > 9 cells. In the double blastocyst transfer cycle, the clinical pregnancy rate showed no significant differences among the groups with 4–5, 6, and 7–9 cells. The implantation and clinical pregnancy rates of D3 embryos with 6 cells significantly decreased; these embryos were not considered as high-quality embryos. Extended culture of D3 embryos with ≤ 6 blastomeres to blastocysts, particularly 6-cell embryos, resulted in a similar clinical pregnancy rate as that of blastocysts derived from D3 embryos with ≥ 7 blastomeres.
登录
查看更多内容
影响因子：
3.1
作者：
Dekel-Naftali, Michal;Aviram-Goldring, Ayala;Rienstein, Shlomit
通讯作者：
Rienstein, Shlomit
卵裂期人类胚胎的细胞数量、分裂行为和发育潜力之间的关系：一项延时研究
影响因子：
3.7
作者：
Kong X;Yang S;Gong F;Lu C;Zhang S;Lu G;Lin G
通讯作者：
Lin G
影响因子：
6.1
作者：
Whitelaw, Natalie;Bhattacharya, Siladitya;Haggarty, Paul
通讯作者：
Haggarty, Paul
影响因子：
6.1
作者：
Martins, W. P.;Nastri, C. O.;Racowsky, C.
通讯作者：
Racowsky, C.
影响因子：
3.1
作者：
Burks, Heather;Buckbinder, Jennifer;Paulson, Richard
通讯作者：
Paulson, Richard