Rapid, direct analysis of cholesterol by charge labeling in reactive desorption electrospray ionization.
Rapid, direct analysis of cholesterol by charge labeling in reactive desorption electrospray ionization.
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DOI：
10.1021/ac901003u
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发表时间：
2009-09-15
影响因子：
7.4
通讯作者：
Cooks, R. Graham
中科院分区：
文献类型：
--
作者：
Wu, Chunping;Ifa, Demian R.;Manicke, Nicholas E.;Cooks, R. Graham
胆固醇的直接和快速分析，在周围环境中使用反应解吸电喷雾电离（DESI）质谱。这是通过在诸如干燥血清样品和动物组织切片的表面上以高速带电液滴的形式电喷雾试剂溶液来实现的。加入到喷雾溶剂中的甜菜碱醛通过亲核加成与胆固醇的醇基团选择性地快速反应，形成半缩醛盐。当将1 μ g/mL胆固醇溶液点在表面上时，纯胆固醇和相关化合物的检测限为1 ng。以胆固醇-d 7为内标，采用反应性DESI定量分析血清中游离胆固醇。高通量分析的小体积的血清点样到一个合适的基板上，实现了在分析速度为10.14秒每个样品，与相对标准偏差（RSD）约为。百分之六在成像模式中使用反应性DESI允许在大鼠脑组织中同时记录磷脂和胆固醇的2D空间分布。
Direct and rapid analysis of cholesterol was accomplished in the ambient environment using reactive desorption electrospray ionization (DESI) mass spectrometry. This was achieved by electrospraying reagent solutions in the form of high velocity charged droplets at surfaces such as dried serum samples and animal tissue sections. Betaine aldehyde, incorporated into the spray solvent, reacts selectively and rapidly with the alcohol group of cholesterol by nucleophilic addition, forming a hemiacetal salt. Limits of detection for pure cholesterol and related compounds were ∼1 ng when a solution of cholesterol of 1ug/mL was spotted onto the surface). Quantitative analysis of free cholesterol in serum using reactive DESI was demonstrated using cholesterol-d7 as internal standard. High throughput analysis of small volumes of serum spotted onto a suitable substrate was achieved at an analysis rate of ∼14 seconds per sample, with a relative standard deviation (RSD) of ca. 6%. Use of reactive DESI in the imaging mode allowed 2D spatial distributions of phospholipids and cholesterol to be recorded simultaneously in rat brain tissues.
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影响因子：
7.4
作者：
Nyadong, Leonard;Green, Michael D.;Fernandez, Facundo M.
通讯作者：
Fernandez, Facundo M.
影响因子：
2
作者：
Ifa, Demian R.;Manicke, Nicholas E.;Cooks, R. Graham
通讯作者：
Cooks, R. Graham
DOI：
10.1002/oms.1210291204
发表时间：
1994-12-01
期刊：
ORGANIC MASS SPECTROMETRY
影响因子：
--
作者：
DOMON, B;MUELLER, DR;RICHTER, WJ
通讯作者：
RICHTER, WJ
影响因子：
7.1
作者：
LEONARD, PJ;SHAPER, AG;JONES, KW
通讯作者：
JONES, KW
影响因子：
2.3
作者：
Quirke, JME;Van Berkel, GJ
通讯作者：
Van Berkel, GJ