Randomized Controlled-Feeding Study of Dietary Emulsifier Carboxymethylcellulose Reveals Detrimental Impacts on the Gut Microbiota and Metabolome.
Randomized Controlled-Feeding Study of Dietary Emulsifier Carboxymethylcellulose Reveals Detrimental Impacts on the Gut Microbiota and Metabolome.
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DOI：
10.1053/j.gastro.2021.11.006
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发表时间：
2022-03
期刊：
影响因子：
29.4
通讯作者：
中科院分区：
文献类型：
--
作者：
流行病学和鼠类研究表明，膳食乳化剂促进与微生物群生态失调相关的疾病的发展。尽管这些化合物对肠道微生物群和肠道健康的有害影响已在动物和体外模型中得到证实，但这些食品添加剂对健康人体的影响仍然很难表征。为了在人类中检验这一概念，我们对普遍存在的合成乳化剂羧甲基纤维素（CMC）进行了一项双盲控制喂养研究，其中健康成年人仅食用不含乳化剂的饮食（n = 9）或每天富含15 g CMC的相同饮食（n = 7），持续11天。相对于对照受试者，CMC消耗适度增加餐后腹部不适，并以减少其多样性的方式扰乱肠道微生物群组成。此外，CMC喂养的受试者表现出粪便代谢组的变化，特别是短链脂肪酸和游离氨基酸的减少。此外，我们确定了2名服用CMC的受试者，他们表现出增加的微生物群侵入正常无菌的内粘液层，这是肠道炎症的核心特征，以及微生物群组成的明显变化。这些结果支持了这样一种观点，即在加工食品中广泛使用CMC可能通过改变肠道微生物组和代谢组而导致一系列慢性炎症性疾病的患病率增加（ClinicalTrials.gov，编号NCT03440229）。
Epidemiologic and murine studies suggest that dietary emulsifiers promote development of diseases associated with microbiota dysbiosis. Although the detrimental impact of these compounds on the intestinal microbiota and intestinal health have been demonstrated in animal and in vitro models, impact of these food additives in healthy humans remains poorly characterized. To examine this notion in humans, we performed a double-blind controlled-feeding study of the ubiquitous synthetic emulsifier carboxymethylcellulose (CMC) in which healthy adults consumed only emulsifier-free diets (n = 9) or an identical diet enriched with 15 g per day of CMC (n = 7) for 11 days. Relative to control subjects, CMC consumption modestly increased postprandial abdominal discomfort and perturbed gut microbiota composition in a way that reduced its diversity. Moreover, CMC-fed subjects exhibited changes in the fecal metabolome, particularly reductions in short-chain fatty acids and free amino acids. Furthermore, we identified 2 subjects consuming CMC who exhibited increased microbiota encroachment into the normally sterile inner mucus layer, a central feature of gut inflammation, as well as stark alterations in microbiota composition. These results support the notion that the broad use of CMC in processed foods may be contributing to increased prevalence of an array of chronic inflammatory diseases by altering the gut microbiome and metabolome (ClinicalTrials.gov, number NCT03440229).
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影响因子：
64.8
作者：
Chassaing B;Koren O;Goodrich JK;Poole AC;Srinivasan S;Ley RE;Gewirtz AT
通讯作者：
Gewirtz AT
影响因子：
3.7
作者：
Chassaing B;Srinivasan G;Delgado MA;Young AN;Gewirtz AT;Vijay-Kumar M
通讯作者：
Vijay-Kumar M
影响因子：
7.4
作者：
Armenta, Jenny M.;Cortes, Diego F.;Pisciotta, John M.;Shuman, Joel L.;Blakeslee, Kenneth;Rasoloson, Dominique;Ogunbiyi, Oluwatosin;Sullivan, David J., Jr.;Shulaev, Vladimir
通讯作者：
Shulaev, Vladimir
DOI：
10.1016/j.jcmgh.2017.04.001
发表时间：
2017-09
影响因子：
7.2
作者：
Chassaing B;Raja SM;Lewis JD;Srinivasan S;Gewirtz AT
通讯作者：
Gewirtz AT
影响因子：
5.1
作者：
Steele, Euridice Martinez;Juul, Filippa;Monteiro, Carlos A.
通讯作者：
Monteiro, Carlos A.