Quantitative analysis of human tissue-specific differences in methylation.
Quantitative analysis of human tissue-specific differences in methylation.
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DOI：
10.1016/j.bbrc.2008.09.044
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发表时间：
2008-11-28
影响因子：
3.1
通讯作者：
Nagase, Hiroki
中科院分区：
文献类型：
--
作者：
Igarashi, Jun;Muroi, Satomi;Kawashima, Hiroyuki;Wang, Xiaofei;Shinojima, Yui;Kitamura, Eiko;Oinuma, Toshinori;Nemoto, Norimichi;Song, Fei;Ghosh, Srimoyee;Held, William A.;Nagase, Hiroki
关键词：
组织特异性差异甲基化区域(TDMRS)在哺乳动物发育和组织分化过程中发挥着不可替代的作用。在这篇报道中，用Sequenom质量阵列对最近确认的小鼠tDMRS的13个人类同源区域的DNA甲基化进行了定量分析，其中亚硫酸氢盐处理的片段利用飞行时间谱分析进行了定量检测。在三个独立个体的不同组织中显示了八个区域。来自8个人的睾丸DNA样本也被分析了甲基化。有趣的是，有证据表明DNA甲基化水平在不同个体之间是不同的。5个睾丸特异性DMR的DNA甲基化水平与精母细胞数量呈显著负相关。然而，位于TRIM38和CASZ1基因附近的tDMR呈正相关。我们的结果表明，tDMRS在小鼠和人之间是保守的，可能在调节组织功能、分化和衰老方面发挥重要作用。
Tissue-specific differentially methylated regions (tDMRs) have been identified and implicated for their indispensable involvement in mammalian development and tissue differentiation. In this report, a quantitative DNA methylation analysis was performed for 13 human orthologous regions of recently confirmed mouse tDMRs by using Sequenom Mass Array, by which bisulfite-treated fragments are quantitatively detected using time of flight masspectroscopy analysis. Eight regions were shown as tDMRs in various tissues from three independent individuals. Testis DNA samples from eight individuals were also analyzed for methylation. Interestingly, there is evidence that the DNA methylation level is divergent among individuals. DNA methylation levels of five testis-specific DMRs were significantly inversely correlated with the number of spermatocytes. However, a positive correlation was seen at tDMRs located near the TRIM38 and CASZ1 genes. Our results indicate that tDMRs are conserved between mouse and human and may have an important role in regulating tissue function, differentiation and aging.
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影响因子：
3.4
作者：
Sardiello M;Cairo S;Fontanella B;Ballabio A;Meroni G
通讯作者：
Meroni G
影响因子：
78.5
作者：
Ushijima, T
通讯作者：
Ushijima, T
DOI：
10.1073/pnas.0607521104
发表时间：
2007-01-02
影响因子：
11.1
作者：
Oakes, C. C.;La Salle, S.;Trasler, J. M.
通讯作者：
Trasler, J. M.
影响因子：
4.4
作者：
Schilling, Elinar;Rehli, Michael
通讯作者：
Rehli, Michael
影响因子：
14.9
作者：
Coolen, Marcel W;Statham, Aaron L;Gardiner-Garden, Margaret;Clark, Susan J
通讯作者：
Clark, Susan J