The asymmetric structure of an icosahedral virus bound to its receptor suggests a mechanism for genome release.
The asymmetric structure of an icosahedral virus bound to its receptor suggests a mechanism for genome release.
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DOI：
10.1016/j.str.2013.05.012
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发表时间：
2013-07-02
期刊：
影响因子：
5.7
通讯作者：
Ranson, Neil A.
中科院分区：
文献类型：
--
作者：
Dent, Kyle C.;Thompson, Rebecca;Barker, Amy M.;Hiscox, Julian A.;Barr, John N.;Stockley, Peter G.;Ranson, Neil A.
简单的球形RNA病毒具有很好理解的对称蛋白质衣壳，但其不对称组分的结构信息很少，例如对感染至关重要的次要蛋白质及其基因组。在这里，我们报告的不对称结构的噬菌体MS 2，连接到它的受体，F-菌毛。这种复合物的冷冻电子断层扫描和亚断层扫描平均导致包装基因组的结构包含清晰的密度，这意味着基因组的构象在每个病毒颗粒中是相同的。数据还表明，单拷贝病毒成熟蛋白破坏了衣壳的对称性，占据了二十面体外壳中由外壳蛋白二聚体填充的位置。因此，这种壳粒可以在受体和基因组结合中发挥其已知的生物学作用，并表明基因组在感染期间的退出途径。描述了病毒受体复合物的不对称结构。在结构中观察到有序基因组RNA的密度。描述了单链RNA病毒与其受体结合的不对称结构如何在保护其基因组的高度对称蛋白质衣壳中显示出独特的特征。这为基因组在感染过程中如何离开其保护容器提供了一个模型。
Simple, spherical RNA viruses have well-understood, symmetric protein capsids, but little structural information is available for their asymmetric components, such as minor proteins and their genomes, which are vital for infection. Here, we report an asymmetric structure of bacteriophage MS2, attached to its receptor, the F-pilus. Cryo-electron tomography and subtomographic averaging of such complexes result in a structure containing clear density for the packaged genome, implying that the conformation of the genome is the same in each virus particle. The data also suggest that the single-copy viral maturation protein breaks the symmetry of the capsid, occupying a position that would be filled by a coat protein dimer in an icosahedral shell. This capsomere can thus fulfill its known biological roles in receptor and genome binding and suggests an exit route for the genome during infection. The asymmetric structure of a virus receptor complex is described The density for ordered genomic RNA was observed in the structure Viral maturation protein was visualized Dent et al. describe how the asymmetric structure of a single-stranded RNA virus bound to its receptor shows a unique feature in the otherwise highly symmetric protein capsid that protects its genome. This suggests a model for how the genome could leave its protective container during infection.
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影响因子：
64.8
作者：
FIERS, W;CONTRERAS, R;YSEBAERT, M
通讯作者：
YSEBAERT, M
影响因子：
5.6
作者：
Bakker, Saskia E.;Ford, Robert J.;Stockley, Peter G.
通讯作者：
Stockley, Peter G.
DOI：
10.1073/pnas.0806786105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Clarke, Margaret;Maddera, Lucinda;Silverman, Philip M.
通讯作者：
Silverman, Philip M.
影响因子：
64.8
作者：
ABADZAPATERO, C;ABDELMEGUID, SS;TSUKIHARA, T
通讯作者：
TSUKIHARA, T
影响因子：
64.8
作者：
Cockburn, JJB;Abrescia, NGA;Stuart, DI
通讯作者：
Stuart, DI