Detection of RNA Modifications by HPLC Analysis and Competitive ELISA

Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
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通过 HPLC 分析和竞争性 ELISA 检测 RNA 修饰

DOI:
10.1007/978-1-4939-0882-0_1
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发表时间:
2014
期刊:
Innate DNA and RNA Recognition
影响因子:
--
通讯作者:
S. Bauer
S. Bauer
中科院分区:
--
文献类型:
--
作者:
A. Kaufmann;S. Bauer

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存在超过100种不同的RNA修饰,这些修饰通过酶在特定的核苷酸位置转录后引入。核糖体RNA(rRNA)和转运RNA(tRNA)表现出最多和最多样化的修饰,可能优化其结构和功能。相反,氧化损伤可导致rRNA和信使RNA(mRNA)的随机修饰,这会严重损害功能。RNA修饰还涉及避免免疫系统的自身RNA识别或病原体的免疫逃避。在这里,我们描述了通过HPLC分析和竞争性ELISA检测RNA修饰。
Over 100 different RNA modifications exist that are introduced posttranscriptionally by enzymes at specific nucleotide positions. Ribosomal RNA (rRNA) and transfer RNA (tRNA) exhibit the most and diverse modifications that presumably optimize their structure and function. In contrast, oxidative damage can lead to random modifications in rRNA and messenger RNA (mRNA) that strongly impair functionality. RNA modifications have also been implicated in avoiding self-RNA recognition by the immune system or immune evasion by pathogens. Here, we describe the detection of RNA modifications by HPLC analysis and competitive ELISA.
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