Novel Ensemble Feature Selection Approach and Application in Repertoire Sequencing Data.
Novel Ensemble Feature Selection Approach and Application in Repertoire Sequencing Data.
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DOI：
10.3389/fgene.2022.821832
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发表时间：
2022
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
T 细胞和 B 细胞组成了适应性免疫系统，主要通过体细胞 V(D)J 基因重组产生。因此，VJ 基因的使用可能是潜在的预后或预测生物标志物。然而，由于组成库的克隆型的异质性，对适应性免疫系统的分析具有挑战性。为了解决 T 和 B 细胞库的异质性，我们提出了一种新颖的集成特征选择方法和专注于 VJ 基因使用的定制统计学习算法。我们将所提出的方法应用于来自康复的 COVID-19 患者和健康捐赠者以及一组接受免疫治疗的肺癌患者的 T 细胞受体序列。我们的方法确定了与健康供体相比，COVID-19 康复患者中使用的不同 VJ 基因，以及与肺癌患者临床反应相关的 VJ 基因。仿真研究表明，集成特征选择方法在效率和准确性方面均优于其他最先进的特征选择方法。它始终具有更高的稳定性和灵敏度以及更低的错误发现率。当与不同的分类方法集成时，集成特征选择方法具有最佳的预测精度。总之，所提出的新方法和整合程序是一种有效的特征选择技术，有助于正确分类不同的亚型，以更好地理解与疾病或治疗相关的适应性免疫反应的特征，从而改进治疗策略。
The T and B cell repertoire make up the adaptive immune system and is mainly generated through somatic V(D)J gene recombination. Thus, the VJ gene usage may be a potential prognostic or predictive biomarker. However, analysis of the adaptive immune system is challenging due to the heterogeneity of the clonotypes that make up the repertoire. To address the heterogeneity of the T and B cell repertoire, we proposed a novel ensemble feature selection approach and customized statistical learning algorithm focusing on the VJ gene usage. We applied the proposed approach to T cell receptor sequences from recovered COVID-19 patients and healthy donors, as well as a group of lung cancer patients who received immunotherapy. Our approach identified distinct VJ genes used in the COVID-19 recovered patients comparing to the healthy donors and the VJ genes associated with the clinical response in the lung cancer patients. Simulation studies show that the ensemble feature selection approach outperformed other state-of-the-art feature selection methods based on both efficiency and accuracy. It consistently yielded higher stability and sensitivity with lower false discovery rates. When integrated with different classification methods, the ensemble feature selection approach had the best prediction accuracy. In conclusion, the proposed novel approach and the integration procedure is an effective feature selection technique to aid in correctly classifying different subtypes to better understand the signatures in the adaptive immune response associated with disease or the treatment in order to improve treatment strategies.
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影响因子：
8.9
作者：
Huang, J;Ling, CX
通讯作者：
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影响因子：
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通讯作者：
KENT, JT
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通讯作者：
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