Comprehensive analysis of TCR repertoire in COVID-19 using single cell sequencing.
Comprehensive analysis of TCR repertoire in COVID-19 using single cell sequencing.
复制标题
使用单细胞测序全面分析COVID-19中的TCR库。
DOI：
10.1016/j.ygeno.2020.12.036
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发表时间：
2021-03
期刊：
影响因子：
4.4
通讯作者：
Jiang Q
中科院分区：
文献类型：
--
作者：
Wang P;Jin X;Zhou W;Luo M;Xu Z;Xu C;Li Y;Ma K;Cao H;Huang Y;Xue G;Jin S;Nie H;Jiang Q
T细胞受体（TCR）在T细胞介导的病毒清除中至关重要。迄今为止，在各种疾病中已经观察到TCR偏倚。然而，缺乏对COVID-19患者TCR库的研究。在这里，我们使用单细胞V（D）J测序对12名COVID-19患者和6名健康对照以及其他病毒感染样本的TCR库进行了比较分析。我们在COVID-19中观察到明显的T细胞克隆扩增。对VJ基因组合的进一步分析显示，在COVID-19患者中，6对VJ基因显著增加，而139对VJ基因显著减少。当同时考虑α和β链的VJ组合时，在COVID-19上频率最高的组合是TRAV 12 -2-J27-TRBV 7 -9-J2-3。此外，在不同病毒感染的样品中也观察到V和J基因片段的优先使用。我们的研究为COVID-19中的TCR提供了新的见解，这有助于我们理解SARS-CoV-2诱导的免疫反应。
T-cell receptor (TCR) is crucial in T cell-mediated virus clearance. To date, TCR bias has been observed in various diseases. However, studies on the TCR repertoire of COVID-19 patients are lacking. Here, we used single-cell V(D)J sequencing to conduct comparative analyses of TCR repertoire between 12 COVID-19 patients and 6 healthy controls, as well as other virus-infected samples. We observed distinct T cell clonal expansion in COVID-19. Further analysis of VJ gene combination revealed 6 VJ pairs significantly increased, while 139 pairs significantly decreased in COVID-19 patients. When considering the VJ combination of α and β chains at the same time, the combination with the highest frequency on COVID-19 was TRAV12-2-J27-TRBV7-9-J2-3. Besides, preferential usage of V and J gene segments was also observed in samples infected by different viruses. Our study provides novel insights on TCR in COVID-19, which contribute to our understanding of the immune response induced by SARS-CoV-2.
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