Splicing of the Sinorhizobium meliloti RmInt1 group II intron provides evidence of retroelement behavior.
Splicing of the Sinorhizobium meliloti RmInt1 group II intron provides evidence of retroelement behavior.
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DOI：
10.1093/nar/gkq847
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发表时间：
2011-02
影响因子：
14.9
通讯作者：
Toro N
中科院分区：
文献类型：
--
作者：
Chillón I;Martínez-Abarca F;Toro N
II族内含子既是大的催化rna，也是可移动的逆转录因子。它们存在于细胞器和细菌基因组中，并通过一个分支中间体剪接，其机制类似于剪接体内含子。然而，它们的分布和插入模式，特别是细菌II群内含子，表明它们的功能和行为更像逆转录因子而不是细胞器内含子。RmInt1是在共生细菌Sinorhizobium meliloti的ISRm2011-2插入序列中发现的一个高效的可移动内含子。这组II内含子在体内和体外作为内含子移植体被切除。然而，完整的剪接反应在体内仍有待阐明。利用lacZ报告基因系统、northern blotting和实时反转录技术研究了RmInt1的剪接活性。拼接效率为0.07±0.02%。这些发现表明细菌II群内含子的功能更像逆转录因子而不是剪接体内含子。它们的位置与这些内含子在防止细菌中其他潜在有害移动元素传播中的作用是一致的。
Group II introns act as both large catalytic RNAs and mobile retroelements. They are found in organelle and bacterial genomes and are spliced via a lariat intermediate, in a mechanism similar to that of spliceosomal introns. However, their distribution and insertion patterns, particularly for bacterial group II introns, suggest that they function and behave more like retroelements than organelle introns. RmInt1 is an efficient mobile intron found within the ISRm2011-2 insertion sequence in the symbiotic bacterium Sinorhizobium meliloti. This group II intron is excised, in vivo and in vitro, as intron lariats. However, the complete splicing reaction in vivo remains to be elucidated. A lacZ reporter gene system, northern blotting and real-time reverse transcription were carried out to investigate RmInt1 splicing activity. Splicing efficiency of 0.07 ± 0.02% was recorded. These findings suggest that bacterial group II introns function more like retroelements than spliceosomal introns. Their location is consistent with a role for these introns in preventing the spread of other potentially harmful mobile elements in bacteria.
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