Antiviral Mechanism of Tea Polyphenols against Porcine Reproductive and Respiratory Syndrome Virus.
Antiviral Mechanism of Tea Polyphenols against Porcine Reproductive and Respiratory Syndrome Virus.
复制标题
茶多酚抗猪繁殖与呼吸综合征病毒的机制
DOI：
10.3390/pathogens10020202
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发表时间：
2021-02-13
期刊：
影响因子：
--
通讯作者：
Guo C
中科院分区：
文献类型：
--
作者：
Wang X;Dong W;Zhang X;Zhu Z;Chen Y;Liu X;Guo C
猪繁殖与呼吸综合征病毒（PRRSV）灭活疫苗和弱毒疫苗均不能有效预防和控制其感染和传播。因此，必须拓宽新的视野，制定有效的预防战略。茶多酚的主要成分是儿茶素及其衍生物。TPP具有多种生理活性，具有一定的抗病毒和抗真菌作用。然而，TPP是否显示抗PRRSV活性仍不清楚。我们发现TPP通过抑制病毒附着、内化、复制和释放的阶段有效地抑制了Marc-145细胞中的PRRSV感染。TPP表现出有效的抗PRRSV效果，无论是治疗前还是治疗后。此外，我们证明TPP抑制PRRSV诱导的p65进入细胞核，抑制NF-κB信号通路的激活，最终导致炎症细胞因子的表达抑制。此外，TPP限制了病毒复制转录复合物的核心组分病毒非结构蛋白2（nsp 2）的合成，这可能有助于抑制病毒RNA复制。TPP有可能成为一种有效的抗病毒药物，用于未来的PRRSV预防和控制。
Neither inactivated nor attenuated vaccines can effectively prevent and control the infection and spread of porcine reproductive and respiratory syndrome virus (PRRSV). Therefore, it is necessary to broaden new horizons and to conceive effective preventive strategies. The main components of Tea polyphenol (TPP) are catechins and their derivatives. TPP has many physiological activities and has certain antiviral and antifungal effects. However, whether TPP shows anti-PRRSV activity remains unclear. We found that TPP effectively inhibited PRRSV infection in Marc-145 cells by suppressing the stages of viral attachment, internalization, replication, and release. TPP exhibited a potent anti-PRRSV effect regardless of pre-treatment or post-treatment. In addition, we demonstrated that TPP restrained PRRSV-induced p65 entry into the nucleus to suppress the activation of the NF-κB signaling pathway, which ultimately leads to the inhibition of the expression of inflammatory cytokines. Furthermore, TPP limited the synthesis of viral non-structural protein 2 (nsp2), the core component of viral replication transcription complexes, which may contribute to the inhibition of viral RNA replication. TPP has the potential to develop into an effective antiviral agent for PRRSV prevention and control in the future.
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影响因子：
5
作者：
Fang Y;Snijder EJ
通讯作者：
Snijder EJ
影响因子：
13.5
作者：
Ciesek, Sandra;von Hahn, Thomas;Steinmann, Eike
通讯作者：
Steinmann, Eike
影响因子：
2.6
作者：
Cho, Yun-Seok;Schiller, Neal L.;Oh, Kye-Heon
通讯作者：
Oh, Kye-Heon
影响因子：
7.1
作者：
Clifford, Michael N.;van der Hooft, Justin J. J.;Crozier, Alan
通讯作者：
Crozier, Alan
影响因子：
2.8
作者：
Cho, Jenny G.;Dee, Scott A.
通讯作者：
Dee, Scott A.