Expression of hunchback during oogenesis and embryogenesis in Locusta migratoria manilensis (Meyen)
Expression of hunchback during oogenesis and embryogenesis in Locusta migratoria manilensis (Meyen)
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马尼拉飞蝗 (Meyen) 卵子发生和胚胎发生过程中驼背的表达
DOI：
10.1007/s11427-010-4128-8
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发表时间：
2011-02
期刊：
影响因子：
--
通讯作者：
CHEN Bin
中科院分区：
文献类型：
--
作者：
HE ZhengBo;LI TingJing;CAO YueQing;CHEN Bin
hb（hunchback）是昆虫的前后轴向模式的一个促成因素。虽然hb在东亚飞蝗中的功能已经被研究，但其表达模式仍然未知。本研究制备了抗血红蛋白融合蛋白的小鼠多克隆抗体，研究了血红蛋白融合蛋白在L.采用免疫组织化学染色法检测东亚飞蝗。血红蛋白蛋白在卵母细胞核中被检测到，位于发育中的卵母细胞的中央。随着卵母细胞的成熟，卵母细胞核沿着逐渐向卵后端移动。在刚产下的卵中，Hb在卵的后端形成梯度，然后在胚盘的中部表达为一条带。随着胚盘向头部和躯干分化，表达区域逐渐扩大，并向空间颌段和胸段发展。随着腹段的出现，在颌部和胸部的表达域逐渐变弱并最终消失，而Hb表达域以不连续的表达方式出现在后部生长区。L.东亚飞蝗（migratoria manilensis）与美洲血吸虫（Schistocerca americana）和东亚飞蝗（L.移民。与其他昆虫相比，hb在颚部和胸部的表达较为保守，而在卵子发生和腹部的表达则有所不同。
hb (hunchback) is a contributing factor in anteroposterior axial patterning of insects. Although the hb function in Locusta migratoria manilensis has been investigated, its expression pattern remains unknown. Here, the mouse polyclonal antibody was produced against Hb fusion protein, and then its expression pattern during oogenesis and embryogenesis of L. migratoria manilensis was examined by immunohistochemical staining. Hb protein was detected in the oocyte nucleus which was positioned centrally within the developing oocyte. The oocyte nucleus gradually moved to the posterior end of the egg along with the oocyte maturing. In freshly laid eggs, Hb formed gradient at the posterior end of the egg, and then hb was expressed as a band in the middle of the blastodisc. As the blastodisc differentiated into the head and trunk, the expression region became wide, which would develop into spatial gnathal and thoracic segments. With abdominal segmentation, the expression domain in the gnathal and thoracic region became faint and eventually faded out, while the Hb expression domain appeared at the posterior growth zone in a discontinuous expression manner. The hb expression pattern of L. migratoria manilensis is greatly similar to that of other locusts, such as Schistocerca americana and another L. migratoria. Compared with other insects, hb expression is conserved in the gnathal and thoracic domains, while divergent in oogenesis and abdomen.
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影响因子：
2.7
作者：
LEHMANN, R;NUSSLEINVOLHARD, C
通讯作者：
NUSSLEINVOLHARD, C
影响因子：
64.8
作者：
Clyde, DE;Corado, MSG;Small, S
通讯作者：
Small, S
影响因子：
2.7
作者：
X. Wu;V. Vasisht;D. Kosman;J. Reinitz;S. Small
通讯作者：
X. Wu;V. Vasisht;D. Kosman;J. Reinitz;S. Small
影响因子：
4.6
作者：
N. Patel;D. Hayward;S. Lall;Nicole Pirkl;Daniel DiPietro;Eldon E. Ball
通讯作者：
N. Patel;D. Hayward;S. Lall;Nicole Pirkl;Daniel DiPietro;Eldon E. Ball
影响因子：
64.8
作者：
D. Tautz;R. Lehmann;H. Schnürch;R. Schuh;E. Seifert;A. Kienlin;K. Jones;H. Jäckle
通讯作者：
D. Tautz;R. Lehmann;H. Schnürch;R. Schuh;E. Seifert;A. Kienlin;K. Jones;H. Jäckle