The natural history of encephalomyocarditis virus-induced myositis and myocarditis in mice. Viral persistence demonstrated by in situ hybridization
The natural history of encephalomyocarditis virus-induced myositis and myocarditis in mice. Viral persistence demonstrated by in situ hybridization
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脑心肌炎病毒引起的小鼠肌炎和心肌炎的自然史。
DOI：
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发表时间：
1988
影响因子：
15.3
通讯作者：
Paul H. Plotz
中科院分区：
文献类型：
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作者：
Cronin Me;Lori A. Love;Frederick W. Miller;Patrick R. McClintock;Paul H. Plotz
小核糖核酸病毒可以引发慢性炎症，这种炎症在无法再从发炎组织中培养病毒后持续存在。为了理解这种转变，我们通过检测病毒基因组的方法来寻找病毒持久性的证据，而与完整的病毒是否存在无关。在感染脑心肌炎病毒肌向变种 EMC-221A 的小鼠中，病毒可在 1 周时从骨骼肌、心脏和大脑中以高产量培养，并在 2 周时以低产量培养； 4周时可从脑中培养出少量斑块形成单位。相比之下，原位杂交至少在一两周后检测到病毒核酸，通常是在单细胞中。在骨骼肌中，炎症在 3 周后消失，但在心脏中，炎症持续了整整 12 周的观察时间。在大脑中，小胶质细胞结节（有时带有相关的病毒核酸）存在很长一段时间。该技术的应用可以更准确地评估病毒持久性在病毒引发但明显的自身免疫性炎症的发病机制中的作用。
Picornaviruses can initiate chronic inflammation that persists after the virus can no longer be cultured from inflamed tissues. In an attempt to understand this transition we have sought evidence for viral persistence by methods that detect viral genome independent of whether or not whole competent virus is present. In mice infected with a myotropic variant of encephalomyocarditis virus, EMC-221A, virus can be cultured in high yield at 1 wk and in low yield at 2 wk from skeletal muscle, heart, and brain; a small number of plaque-forming units could be cultured from brain at 4 wk. By contrast, in situ hybridization detected viral nucleic acid at least a week or two thereafter, often in single cells. In the skeletal muscle, inflammation disappeared by 3 wk, but in heart it remained for the full 12 wk of observation. In the brain, microglial nodules, sometimes with associated viral nucleic acid, were present for a long period. Application of this technique allows a more accurate assessment of the role of viral persistence in the pathogenesis of virus-initiated but apparently autoimmune inflammation.
福尔马林固定石蜡包埋的脑组织切片与适合原位杂交的载玻片共价结合。
影响因子：
3.1
作者：
Tourtellotte,WW;Verity,AN;Schmid,P;Martinez,S;Shapshak,P
通讯作者：
Shapshak,P
泰勒氏鼠脑脊髓炎病毒 (TMEV) 诱导的脱髓鞘疾病中 II 类限制性 T 细胞反应。
DOI：
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发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Miller,SD;Clatch,RJ;Pevear,DC;Trotter,JL;Lipton,HL
通讯作者：
Lipton,HL
影响因子：
14.9
作者：
PALMENBERG, AC;KIRBY, EM;COLLETT, MS
通讯作者：
COLLETT, MS