The 2009 pandemic (H1N1) viruses isolated from pigs show enhanced pathogenicity in mice.
The 2009 pandemic (H1N1) viruses isolated from pigs show enhanced pathogenicity in mice.
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从猪中分离出的 2009 年大流行 (H1N1) 病毒对小鼠的致病性增强
DOI：
10.1186/1297-9716-44-41
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发表时间：
2013-06-11
影响因子：
4.4
通讯作者：
Jin M
中科院分区：
文献类型：
--
作者：
Li Y;Zou W;Jia G;Ke J;Zhu J;Lin X;Zhou H;Jin M
自2009年4月出现2009年大流行性（H1N1）病毒（2009/H1N1）以来，经常报告人向猪传播的病例。在我们以前的研究中，从猪中分离出四种2009/H1N1变种。为了更好地了解猪分离株与人分离株的表型差异，本研究在小鼠鼻内接种了不同的2009/H1N1病毒，并监测了发病率、死亡率、病毒复制、细胞因子产生和肺部病理变化。结果表明，所有分离株都能在肺中有效复制，但其致病能力各不相同。其中，A/swine/南昌/3/2010 （H1N1）和A/swine/南昌/F9/2010 （H1N1）毒株毒力最强，在小鼠中持续复制，致死率高，而人分离株A/辽宁/14/2009 （H1N1）毒株对小鼠的毒力较低。与其他菌株相比，致死菌株在早期可引起更严重的肺病理改变和更高的细胞因子产生。氨基酸序列分析表明猪分离株和人分离株在病毒表面糖蛋白和聚合酶亚基上存在显著差异，这可能与它们的表型差异有关。这些研究表明，2009/H1N1猪分离株在小鼠中表现出异质传染性和致病性，并且与人分离株相比，某些菌株具有增强的致病性。
Since the emergence of the 2009 pandemic (H1N1) virus (2009/H1N1) in April 2009, cases of transmission from humans to pigs have been reported frequently. In our previous studies, four 2009/H1N1 variants were isolated from pigs. To better understand the phenotypic differences of the pig isolates compared with the human isolate, in this study mice were inoculated intranasally with different 2009/H1N1 viruses, and monitored for morbidity, mortality, and viral replication, cytokine production and pathological changes in the lungs. The results show that all isolates show effective replication in lungs, but varying in their ability to cause morbidity. In particular, the strains of A/swine/Nanchang/3/2010 (H1N1) and A/swine/Nanchang/F9/2010 (H1N1) show the greatest virulence with a persisting replication in lungs and high lethality for mice, compared with the human isolate A/Liaoning /14/2009 (H1N1), which shows low virulence in mice. Furthermore, the lethal strains could induce more severe lung pathological changes and higher production of cytokines than that of other strains at an early stage. Amino acid sequence analysis illustrates prominent differences in viral surface glycoproteins and polymerase subunits between pig isolates and human strains that might correlate with their phenotypic differences. These studies demonstrate that the 2009/H1N1 pig isolates exhibit heterogeneous infectivity and pathogencity in mice, and some strains possess an enhanced pathogenicity compared with the human isolate.
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DOI：
10.1086/656365
发表时间：
2010-10-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Perrone LA;Szretter KJ;Katz JM;Mizgerd JP;Tumpey TM
通讯作者：
Tumpey TM
影响因子：
11.8
作者：
Kalthoff D;Grund C;Harder TC;Lange E;Vahlenkamp TW;Mettenleiter TC;Beer M
通讯作者：
Beer M
影响因子：
11.8
作者：
Sreta D;Tantawet S;Na Ayudhya SN;Thontiravong A;Wongphatcharachai M;Lapkuntod J;Bunpapong N;Tuanudom R;Suradhat S;Vimolket L;Poovorawan Y;Thanawongnuwech R;Amonsin A;Kitikoon P
通讯作者：
Kitikoon P
影响因子：
5.4
作者：
Mehle, Andrew;Dugan, Vivien G.;Doudna, Jennifer A.
通讯作者：
Doudna, Jennifer A.
影响因子：
11.8
作者：
Howard, Wendy A.;Essen, Steve C.;Brown, Ian H.
通讯作者：
Brown, Ian H.