A novel pathway of intercellular signalling in Bacillus subtilis involves a protein with similarity to a component of type III secretion channels.
A novel pathway of intercellular signalling in Bacillus subtilis involves a protein with similarity to a component of type III secretion channels.
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DOI：
10.1111/j.1365-2958.2008.06289.x
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发表时间：
2008-07
影响因子：
3.6
通讯作者：
Losick R
中科院分区：
文献类型：
--
作者：
Camp AH;Losick R
在枯草芽孢杆菌孢子形成过程中，孢子囊母细胞区室中σE介导的基因表达通过由多种功能未知的蛋白质组成的细胞间信号传导途径触发前孢子中σG的激活。在这里，我们确认了在 σE 控制下在母细胞中产生的营养蛋白 SpoIIIJ、前孢子蛋白 SpoIIQ 和八种膜蛋白（SpoIIIAA 至 SpoIIIAH）通常是细胞间信号传导所必需的。相比之下，先前与该通路有关的抗 σG 因子被证明是可有可无的。我们还提供了证据表明 SpoIIIJ 是一种膜蛋白转位酶，可促进 SpoIIIAE 插入膜中。此外，我们报告了在 σG 激活中分离出的突变，该突变通过 SpoIIIAG 部分绕过了 SpoIIIJ 和 SpoIIIAA 的要求，但不绕过 SpoIIIAH 或 SpoIIQ 的要求。因此，我们提出在某些遗传条件下，SpoIIIAH和SpoIIQ可以构成激活σG的最小途径。最后，基于 SpoIIIAH 与 III 型分泌系统组件的相似性，我们推测信号传导是由连接母细胞与前孢子的通道介导的。
During spore formation in Bacillus subtilis, σE-directed gene expression in the mother-cell compartment of the sporangium triggers the activation of σG in the forespore by a pathway of intercellular signaling that is composed of multiple proteins of unknown function. Here, we confirm that the vegetative protein SpoIIIJ, the forespore protein SpoIIQ, and eight membrane proteins (SpoIIIAA through SpoIIIAH) produced in the mother cell under the control of σE are ordinarily required for intercellular signaling. In contrast, an anti-σG factor previously implicated in the pathway is shown to be dispensable. We also present evidence suggesting that SpoIIIJ is a membrane protein translocase that facilitates the insertion of SpoIIIAE into the membrane. In addition, we report the isolation of a mutation that partially bypasses the requirement for SpoIIIJ and for SpoIIIAA through SpoIIIAG, but not for SpoIIIAH or SpoIIQ, in the activation of σG. We therefore propose that under certain genetic conditions, SpoIIIAH and SpoIIQ can constitute a minimal pathway for the activation of σG. Finally, based on the similarity of SpoIIIAH to a component of type III secretion systems, we speculate that signaling is mediated by a channel that links the mother cell to the forespore.
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影响因子：
3.6
作者：
Crepin, VF;Prasannan, S;Matthews, S
通讯作者：
Matthews, S
影响因子：
3.2
作者：
McPherson, DC;Driks, A;Popham, DL
通讯作者：
Popham, DL
影响因子：
14.9
作者：
Käll L;Krogh A;Sonnhammer EL
通讯作者：
Sonnhammer EL
影响因子：
3.2
作者：
FRANDSEN, N;STRAGIER, P
通讯作者：
STRAGIER, P
影响因子：
10.5
作者：
Blaylock, B;Jiang, X;Pogliano, K
通讯作者：
Pogliano, K