Guidelines for Biobanking of Bone Marrow Adipose Tissue and Related Cell Types: Report of the Biobanking Working Group of the International Bone Marrow Adiposity Society.
Guidelines for Biobanking of Bone Marrow Adipose Tissue and Related Cell Types: Report of the Biobanking Working Group of the International Bone Marrow Adiposity Society.
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DOI：
10.3389/fendo.2021.744527
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发表时间：
2021
影响因子：
5.2
通讯作者：
van der Eerden BCJ
中科院分区：
文献类型：
--
作者：
Lucas S;Tencerova M;von der Weid B;Andersen TL;Attané C;Behler-Janbeck F;Cawthorn WP;Ivaska KK;Naveiras O;Podgorski I;Reagan MR;van der Eerden BCJ
在过去的二十年里，科学家们对研究骨髓脂肪(BMA)与骨和脂肪组织生理学的关系的兴趣与日俱增，增加了使用不同来源的骨髓脂肪组织(BMAT)的出版物数量。然而，每种来源的BMAT在可用于下游分析的数量上都有其局限性。基于这一日益增长的科学需求，国际骨髓脂肪学会(BMAS)成立了一个生物库工作组，以确定人类BMA相关样本生物库的挑战，并制定指导方针，以推动为BMA研究建立生物库。BMA是一个年轻的、不断增长的领域，许多不同的科学界对此越来越感兴趣。这些都带来了新的视角和重要的生物学问题，即如何改善和建立一个拥有可在世界各地使用和共享的生物库数据库的国际社会。然而，为了建立可在国际上使用的生物库，必须解决几个实际和立法问题，以制定一项在所有机构中使用的一般伦理协议，以便在国际上交换生物材料。在这份立场文件中，BMAS生物库工作组描述了来自不同机构的患者信息(PIF)和同意书的异同，并讨论了为BMA生物库目的创建统一文件的可能性。此外，在工作组成员讨论的基础上，我们报告了目前人骨髓脂肪细胞(BMAd)和骨髓基质细胞(BMSCs，以前称为间充质细胞)分离方案的概述，重点介绍了有效分离的关键要点。虽然我们还远没有一个统一的BMAD分离协议和PIF，但我们已经总结了建立BMA生物库所需的所有这些重要方面。总之，我们相信，在全球范围内协调隔离协议和PIF将有助于建立国际合作，并提高BMA研究成果的质量和解释。
Over the last two decades, increased interest of scientists to study bone marrow adiposity (BMA) in relation to bone and adipose tissue physiology has expanded the number of publications using different sources of bone marrow adipose tissue (BMAT). However, each source of BMAT has its limitations in the number of downstream analyses for which it can be used. Based on this increased scientific demand, the International Bone Marrow Adiposity Society (BMAS) established a Biobanking Working Group to identify the challenges of biobanking for human BMA-related samples and to develop guidelines to advance establishment of biobanks for BMA research. BMA is a young, growing field with increased interest among many diverse scientific communities. These bring new perspectives and important biological questions on how to improve and build an international community with biobank databases that can be used and shared all over the world. However, to create internationally accessible biobanks, several practical and legislative issues must be addressed to create a general ethical protocol used in all institutes, to allow for exchange of biological material internationally. In this position paper, the BMAS Biobanking Working Group describes similarities and differences of patient information (PIF) and consent forms from different institutes and addresses a possibility to create uniform documents for BMA biobanking purposes. Further, based on discussion among Working Group members, we report an overview of the current isolation protocols for human bone marrow adipocytes (BMAds) and bone marrow stromal cells (BMSCs, formerly mesenchymal), highlighting the specific points crucial for effective isolation. Although we remain far from a unified BMAd isolation protocol and PIF, we have summarized all of these important aspects, which are needed to build a BMA biobank. In conclusion, we believe that harmonizing isolation protocols and PIF globally will help to build international collaborations and improve the quality and interpretation of BMA research outcomes.
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