Influence of Altered NADH Metabolic Pathway on the Respiratory-deficient Mutant of Rhizopus oryzae and its L-lactate Production
Influence of Altered NADH Metabolic Pathway on the Respiratory-deficient Mutant of Rhizopus oryzae and its L-lactate Production
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NADH代谢途径改变对米根霉呼吸缺陷突变体及其L-乳酸生产的影响
DOI：
10.1007/s12010-015-1700-5
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发表时间：
2015-06
影响因子：
3
通讯作者：
Zheng, Zhi
中科院分区：
文献类型：
--
作者：
Guo, Chenchen;Luo, Shuizhong;Jiang, Shaotong;Zheng, Zhi
Respiratory-deficient mutants of Rhizopus oryzae (R. oryzae) AS 3.3461 were acquired by ultraviolet (UV) irradiation to investigate changes in intracellular NADH metabolic pathway and its influence on the fermentation characteristics of the strain. Compared with R. oryzae AS 3.3461, the intracellular ATP level of the respiratory-deficient strain UV-1 decreased by 52.7 % and the glucose utilization rate rose by 8.9 %; When incubated for 36 h, the activities of phosphofructokinase (PFK), hexokinase (HK), and pyruvate kinase (PK) in the mutant rose by 74.2, 7.2, and 12.0 %, respectively; when incubated for 48 h, the intracellular NADH/NAD+ ratio of the mutant rose by 14.6 %; when a mixed carbon source with a glucose/gluconic acid ratio of 1:1 was substituted to culture the mutant, the NADH/NAD+ ratio decreased by 4.6 %; the ATP content dropped by 27.6 %; the lactate dehydrogenase (LDH) activity rose by 22.7 %; and the lactate yield rose by 11.6 %. These results indicated that changes to the NADH metabolic pathway under a low-energy charge level can effectively increase the glycolytic rate and further improve the yield of L-lactate of R. oryzae.
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DOI：
--
发表时间：
2010
期刊：
Journal of the South China Agricultural University
影响因子：
--
作者：
Zhao Chuan;Lin Qingsheng;Yang Liu;Hu Meiying
通讯作者：
Zhao Chuan;Lin Qingsheng;Yang Liu;Hu Meiying
影响因子：
1.5
作者：
Amanda K. Pearce;Kay Crimmins;E. Groussac;Michael J. E. Hewlins;J. Richard Dickinson;Jean Francois;Ian R. Booth;Alistair J. P. Brown
通讯作者：
Amanda K. Pearce;Kay Crimmins;E. Groussac;Michael J. E. Hewlins;J. Richard Dickinson;Jean Francois;Ian R. Booth;Alistair J. P. Brown
DOI：
--
发表时间：
2008
期刊：
Food Science
影响因子：
--
作者：
Gu Chun-yan
通讯作者：
Gu Chun-yan
影响因子：
3
作者：
S. Thitiprasert;P. Songserm;Wasinee Boonkong;S. Sooksai;K. Kodama;N. Thongchul
通讯作者：
S. Thitiprasert;P. Songserm;Wasinee Boonkong;S. Sooksai;K. Kodama;N. Thongchul
DOI：
10.1152/ajpendo.00054.2012
发表时间：
2012-08
期刊：
American journal of physiology. Endocrinology and metabolism
影响因子：
--
作者：
Amanda T. White;S. Schenk
通讯作者：
Amanda T. White;S. Schenk