Differential distribution of phospholipase C beta isoforms and diaglycerol kinase-beta in rodents cerebella corroborates the division of unipolar brush cells into two major subtypes.
Differential distribution of phospholipase C beta isoforms and diaglycerol kinase-beta in rodents cerebella corroborates the division of unipolar brush cells into two major subtypes.
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DOI：
10.1007/s00429-013-0531-9
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发表时间：
2014-03
影响因子：
3.1
通讯作者：
Mugnaini, Enrico
中科院分区：
文献类型：
--
作者：
Sekerkova, Gabriella;Watanabe, Masahiko;Martina, Marco;Mugnaini, Enrico
关键词：
Sublineage diversification of specific neural cell classes occurs in complex as well as simply organized regions of the central and peripheral nervous systems; the significance of the phenomenon, however, remains insufficiently understood. The unipolar brush cells (UBCs) are glutamatergic cerebellar interneurons that occur at high density in vestibulocerebellum. As they are classified into subsets that differ in chemical phenotypes, intrinsic properties, and lobular distribution, they represent a valuable neuronal model to study subclass diversification. In this study we show that cerebellar UBCs of adult rats and mice form two subclasses – type I and type II UBCs - defined by somatodendritic expression of calretinin (CR), mGluR1α, phospholipases PLCβ1 and PLCβ4, and diacylglycerol kinase-beta (DGKβ). We demonstrate that PLCβ1 is associated only with the CR+ type I UBCs while PLCβ4 and DGKβ are exclusively present in mGluR1α+ type II UBCs. Notably, all PLCβ4+ UBCs, representing about 2/3 of entire UBC population, also express mGluR1α. Furthermore, our data show that the sum of CR+ type I UBCs and mGluR1α+ type II UBCs accounts for the entire UBC class identified with Tbr2 immunolabeling. The two UBC subtypes also show a very different albeit somehow overlapping topographical distribution as illustrated by detailed cerebellar maps in this study. Our data not only complement and extend the previous knowledge on the diversity and subclass specificity of the chemical phenotypes within the UBC population but provide a new angle to the understanding of the signaling networks in type I and type II UBCs.
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影响因子：
3.3
作者：
Chung, S. -H.;Sillitoe, R. V.;Hawkes, R.
通讯作者：
Hawkes, R.
影响因子：
2
作者：
Diño, MR;Perachio, AA;Mugnaini, E
通讯作者：
Mugnaini, E
影响因子：
3.4
作者：
Hozumi, Yasukazu;Fukaya, Masahiro;Goto, Kaoru
通讯作者：
Goto, Kaoru
影响因子：
64.8
作者：
Kim, D;Jun, KS;Shin, HS
通讯作者：
Shin, HS
DOI：
10.1023/a:1007072105919
发表时间：
1999-02-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Diño, MR;Willard, FH;Mugnaini, E
通讯作者：
Mugnaini, E