Expression and function of murine receptor tyrosine kinases, TIE and TEK, in hematopoietic stem cells.
Expression and function of murine receptor tyrosine kinases, TIE and TEK, in hematopoietic stem cells.
复制标题
小鼠受体酪氨酸激酶 TIE 和 TEK 在造血干细胞中的表达和功能。
作者：
M. Yano;A. Iwama;Hitoshi Nishio;J. Suda;G. Takada;T. Suda
两种高度相关的受体酪氨酸激酶 TIE 和 TEK 构成内皮细胞特异性激酶家族。我们建立了针对它们的单克隆抗体，并对它们在小鼠造血干细胞（HSC）中的表达和功能进行了详细分析。 TIE 和 TEK 在 23.7% 和 33.3% 的谱系标记阴性、c-Kit+ 和 Sca-1+ (Lin-c-Kit+ Sca-1+) HSC 上表达，这些 HSC 包含大部分第 12 天集落形成单位-脾 (CFU-S) 和长期重建细胞，但不包含定型祖细胞。 Lin-c-Kit+ Sca-1+ 细胞根据 TIE 和 TEK 的表达进一步划分。 TIE+和TEK+ HSC以及每个阴性对应物均含有高增殖潜力的集落形成细胞，并在体外和体内分化为淋巴和骨髓后代。然而，第 12 天的 CFU-S 在 TIE+ 和 TEK+ HSC 中富集。我们的研究结果将 TIE 和 TEK 定义为分离第 12 天 CFU-S 的新型干细胞标记抗原，并提供了参与 HSC 在特定分化阶段（特别是第 12 天 CFU-S）功能调节的新型信号传导途径的证据。
Two highly related receptor tyrosine kinases, TIE and TEK, comprise a family of endothelial cell-specific kinase. We established monoclonal antibodies against them and performed detailed analyses on their expression and function in murine hematopoietic stem cells (HSCs). TIE and TEK were expressed on 23.7% and 33.3% of lineage marker-negative, c-Kit+ and Sca-1+ (Lin- c-Kit+ Sca-1+) HSCs that contain the majority of day-12 colony-forming units-spleen (CFU-S) and long-term reconstituting cells, but not committed progenitor cells. Lin- c-Kit+ Sca-1+ cells were further divided by the expression of TIE and TEK. TIE+ and TEK+ HSCs as well as each negative counterpart contained high proliferative potential colony-forming cells and differentiated into lymphoid and myeloid progenies both in vitro and in vivo. However, day-12 CFU-S were enriched in TIE+ and TEK+ HSCs. Our findings define TIE and TEK as novel stem cell marker antigens that segregate day-12 CFU-S, and provide evidence of novel signaling pathways that are involved in the functional regulation of HSCs at a specific stage of differentiation, particularly of day-12 CFU-S.
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影响因子：
20.3
作者：
M. Ogawa
通讯作者：
M. Ogawa
DOI：
10.1073/pnas.89.4.1502
发表时间：
1992-02-15
影响因子：
11.1
作者：
IKUTA, K;WEISSMAN, IL
通讯作者：
WEISSMAN, IL
影响因子：
32.4
作者：
MORRISON, SJ;WEISSMAN, IL
通讯作者：
WEISSMAN, IL
影响因子：
32.4
作者：
MACKAREHTSCHIAN, K;HARDIN, JD;LEMISCHKA, IR
通讯作者：
LEMISCHKA, IR