First Report of Mitochondrial DNA Copy Number Variation in Opsius heydeni (Insecta, Hemiptera, Cicadellidae) from Polluted and Control Sites.
First Report of Mitochondrial DNA Copy Number Variation in Opsius heydeni (Insecta, Hemiptera, Cicadellidae) from Polluted and Control Sites.
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DOI：
10.3390/ani13111793
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发表时间：
2023-05-28
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影响因子：
--
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工业活动由于释放各种污染物而严重破坏周围环境。因此，寻找能够提供环境质量信息的参数具有重要意义。在这项研究中，第一次，属于Opsius heydeni，一种生活在柽柳植物上的昆虫，在西西里岛的两个地点（污染的工业场地Priolo加加洛和对照场地Oasi del Simeto）进行了采样和分析，以研究污染对野生生物的影响。利用分子生物学技术，已经发现环境污染物可以改变这些标本中的线粒体DNA;特别是在Priolo加加洛采样的标本中观察到DNA分子数量减少。总之，这些结果突出了所研究的物种如何有助于监测污染物对生物区系的影响。线粒体DNA容易由于暴露于应激因素而发生改变。特别是，线粒体DNA拷贝数（mtDNAcn）的变化可以用作暴露于各种环境污染物的影响的生物标志物。在这项研究中，mtDNAcn变异的评价的基础上的分子调查首次适用于个人属于物种Opsius heydeni。从西西里东部的两个地点共收集了20个样本：Priolo加加洛，一个具有强烈人类影响的地点，以及Simeto河绿洲，一个对照地点。根据形态特征鉴定的标本用于获得先前在GenBank中不可用的该物种的COI基因序列。处理后，使用COI和18 S基因的一部分的实时PCR评估相对mtDNAcn。在污染现场的标本中观察到的mtDNAAcn的减少。这些结果突出了环境污染物如何改变线粒体基因组以及Opsius heydeni如何被认为是环境质量的潜在生物指示物种。
Industrial activities significantly damage the surrounding environment due to the release of contaminants of various types. Therefore, the search for parameters that can provide information on the quality of the environment is of great relevance. In this research, for the first time, specimens belonging to Opsius heydeni, an insect living on Tamarix plants, were sampled and analyzed at two Sicilian sites (the polluted industrial site Priolo Gargallo and the control site Oasi del Simeto) to study the effect of pollution on wildlife organisms. Using molecular biology techniques, it has been seen that environmental pollutants can modify the mitochondrial DNA in these specimens; in particular, a decrease in the number of DNA molecules was observed in the specimens sampled at Priolo Gargallo. In conclusion, these results highlight how the studied species can be useful to monitor the effects of pollutants on biota. Mitochondrial DNA easily undergoes alterations due to exposure to stress factors. In particular, mitochondrial DNA copy number (mtDNAcn) variation can be used as a biomarker of the effect of exposure to various environmental contaminants. In this study, a molecular investigation based on the evaluation of mtDNAcn variation was applied for the first time to individuals belonging to the species Opsius heydeni. A total of 20 samples were collected from two sites in eastern Sicily: Priolo Gargallo, a site with a strong anthropic impact, and the Simeto river Oasis, a control site. Specimens identified based on morphological traits were used to obtain COI gene sequences from this species that were not previously available in GenBank. After processing, the relative mtDNAcn was evaluated using real-time PCR of a portion of the COI and 18S genes. A decrease in the mtDNAcn in the specimens from the polluted site was observed. These results highlight how environmental contaminants can alter the mitochondrial genome and how Opsius heydeni can be considered a potential bioindicator species of environmental quality.
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