Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D₃ or D₂supplementation.
Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D₃ or D₂supplementation.
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DOI：
10.1186/1475-2891-12-39
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发表时间：
2013-04-04
影响因子：
5.4
通讯作者：
Ongphiphadhanakul B
中科院分区：
文献类型：
--
作者：
Nimitphong H;Saetung S;Chanprasertyotin S;Chailurkit LO;Ongphiphadhanakul B
目前尚不清楚维生素D结合蛋白（DBP）的遗传变异是否会影响维生素D补充后的25-羟基维生素D水平[25（OH）D]。我们的目的是调查泰国队列中总25（OH）D，25（OH）D3和25（OH）D2的变化，根据维生素D补充剂的类型（维生素D3或D2）和DBP基因型，在接受维生素D3或D2 3个月后。39名健康受试者完成了研究。所有受试者每天接受400 IU的维生素D3或D2，加上钙补充剂，持续3个月。通过LC-MS/MS测量血清总25（OH）D、25（OH）D3和25（OH）D2。使用实时PCR进行DBP基因中rs 4588的个体基因分型。补充400 IU/d的维生素D3显著增加25（OH）D3（+16.2 ± 4.2 nmol/L，p <0.001）。维生素D2（400 IU/d）导致25（OH）D2水平升高（+22.0 ± 2.11 nmol/L，p <0.001），同时25（OH）D3水平降低（-14.2 ± 2.0 nmol/L，p <0.001）。3个月时，维生素D3组受试者的总25（OH）D水平往往高于维生素D2组（67.8 ± 3.9 vs. 61.0 ± 3.0 nmol/L; p = 0.08）。然后将受试者分为两个亚组：DBP rs 4588 C等位基因纯合子（CC）和其余（CA或AA）。补充D3后，与CC等位基因相比，CA或AA等位基因受试者的25（OH）D3和总25（OH）D增加显著较少。然而，当补充剂是维生素D2时，没有发现差异。DBP的遗传变异（rs 4588 SNP）影响对维生素D3的反应性，但不影响对维生素D2的反应性。
It is not known whether genetic variation in the vitamin D binding protein (DBP) influences 25-hydroxyvitamin D levels [25(OH)D] after vitamin D supplementation. We aimed to investigate the changes of total 25(OH)D, 25(OH)D3 and 25(OH)D2 in a Thai cohort, according to type of vitamin D supplement (vitamin D3 or D2) and DBP genotype, after receiving vitamin D3 or D2 for 3 months. Thirty-nine healthy subjects completed the study. All subjects received 400 IU of either vitamin D3 or D2, plus a calcium supplement, every day for 3 months. Total serum 25(OH)D, 25(OH)D3 and 25(OH)D2 were measured by LC-MS/MS. Individual genotyping of rs4588 in the DBP gene was performed using real-time PCR. Vitamin D3 supplementation of 400 IU/d increased 25(OH)D3 significantly (+16.2 ± 4.2 nmol/L, p <0.001). Vitamin D2 (400 IU/d) caused increased 25(OH)D2 levels (+22.0 ± 2.11 nmol/L, p <0.001), together with a decrease of 25(OH)D3 (−14.2 ± 2.0 nmol/L, p <0.001). At 3 month, subjects in vitamin D3 group tended to have higher total 25(OH)D levels than those in vitamin D2 (67.8 ± 3.9 vs. 61.0 ± 3.0 nmol/L; p = 0.08). Subjects were then classified into two subgroups: homozygous for the DBP rs4588 C allele (CC), and the rest (CA or AA). With D3 supplementation, subjects with CA or AA alleles had significantly less increase in 25(OH)D3 and total 25(OH)D when compared with those with the CC allele. However, no difference was found when the supplement was vitamin D2. Genetic variation in DBP (rs4588 SNP) influences responsiveness to vitamin D3 but not vitamin D2.
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DOI：
10.1016/0022-4731(84)90063-3
发表时间：
1984-01-01
影响因子：
4.1
作者：
HOLLIS, BW
通讯作者：
HOLLIS, BW
影响因子：
4.1
作者：
Glendenning, Paul;Chew, Gerard T.;Fraser, William D.
通讯作者：
Fraser, William D.
影响因子：
15.9
作者：
Safadi, FF;Thornton, P;Cooke, NE
通讯作者：
Cooke, NE
影响因子：
7.1
作者：
Sinotte, Marc;Diorio, Caroline;Brisson, Jacques
通讯作者：
Brisson, Jacques
影响因子：
5.8
作者：
BIKLE, DD;GEE, E;HADDAD, JG
通讯作者：
HADDAD, JG