Gene expression during Drosophila melanogaster oogenesis: analysis by in situ hybridization to tissue sections.
Gene expression during Drosophila melanogaster oogenesis: analysis by in situ hybridization to tissue sections.
复制标题
果蝇卵子发生过程中的基因表达：通过组织切片原位杂交进行分析。
DOI：
10.1016/0012-1606(84)90263-x
复制
发表时间：
1984
影响因子：
2.7
通讯作者：
P. Schedl
中科院分区：
文献类型：
--
作者：
L. Ambrosio;P. Schedl
在卵巢切片制备中，通过RNA原位杂交检测了卵子发生过程中特定基因的基因表达模式。热休克前后与热休克基因互补的RNA积累(1)；(2)与三个λ噬菌体重组体互补的RNA，这些噬菌体重组体含有卵发生过程中表达的序列；(3)分析了与治疗基因互补的RNA。基因在卵巢中的表达被发现依赖于细胞类型和卵巢腔的发育阶段。在哺乳细胞中，卵黄形成的开始（8-11期）似乎伴随着许多不同基因的大量激活，并且互补rna的积累水平显著增加。此外，乳母细胞在卵黄形成过程中合成的许多RNA最终被运送到生长中的卵母细胞中并在那里积累。这种RNA的积累很可能为胚胎发生提供了基因产物的来源，无论是作为信息还是蛋白质。
The pattern of gene expression for specific genes during oogenesis has been examined byin situhybridization to RNA in sectioned ovarian preparations. The accumulation (1) of RNA complementary to heat-shock genes, both before and after heat shock; (2) of RNA complementary to three λ phage recombinants which contain sequences expressed during oogenesis; and (3) of RNA complementary to therudimentarygene have been analyzed. Gene expression in the ovaries is found to be dependent on both the cell type and the developmental stage of the ovarian chamber. In the nurse cells, the onset of vitellogenesis (stages 8–11) appears to be accompanied by a substantial activation of many different genes and there is a pronounced increase in the level of accumulation of complementary RNAs. Moreover, much of the RNA synthesized in nurse cells during vitellogenesis is ultimately transported into the growing oocyte where it accumulates. It is likely that this accumulation of RNA provides a source of gene products, either as message or proteins, for embryogenesis.
影响因子：
5.6
作者：
Segraves,WA;Louis,C;Tsubota,S;Schedl,P;Rawls,JM;Jarry,BP
通讯作者：
Jarry,BP