Effects of anticoagulants on human plasma soluble corin levels measured by ELISA.
Effects of anticoagulants on human plasma soluble corin levels measured by ELISA.
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DOI：
10.1016/j.cca.2010.08.021
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发表时间：
2010-12-14
期刊：
影响因子：
--
通讯作者：
Wu Q
中科院分区：
文献类型：
--
作者：
Dong N;Dong J;Liu P;Xu L;Shi S;Wu Q
最近，在人血浆中检测到可溶性corin。心力衰竭患者血浆corin水平低于正常对照组。在这项研究中，我们分析了测定血浆或血清corin的免疫测定的实验条件。ELISA法测定血清和血浆corin水平。研究了不同抗凝剂（EDTA、肝素和柠檬酸钠）对血浆corin水平的影响。血清中Corin水平与肝素血浆中相似（950 ± 305对929 ± 301 pg/ml，n=40，p=0.73），但显著高于柠檬酸钠（735 ± 237 pg/ml，p<0.01）或EDTA（716 ± 261 pg/ml，p<0.001）血浆中的Corin水平。天然和重组人corin蛋白在人血浆中与EDTA在4°C下稳定或经历冻融。在348名健康中国人中，血浆corin水平范围为216 - 1663 pg/ml。男性高于女性（842 ± 283 vs. 569 ± 192 pg/ml，p<0.001）。可溶性可林在血浆样品中稳定。血浆可溶性corin水平因所用抗凝剂而异。含有肝素的样品中corin的水平显著高于含有EDTA或柠檬酸钠的样品中corin的水平。
Recently, soluble corin was detected in human plasma. In patients with heart failure, plasma corin levels were lower than that of normal controls. In this study, we analyzed experimental conditions for measuring plasma or serum corin by an immunoassay. Serum and plasma corin levels were measured by ELISA. Effects of different anticoagulants (EDTA, heparin and sodium citrate) on plasma corin levels were examined. Corin levels in serum were similar to that in plasma with heparin (950 ± 305 vs. 929 ± 301 pg/ml, n=40, p=0.73), but were significantly higher than those in plasma with sodium citrate (735 ± 237 pg/ml, p<0.01) or EDTA (716 ± 261 pg/ml, p<0.001). Native and recombinant human corin proteins were stable in human plasma with EDTA at 4°C or underwent freezing-and-thawing. In 348 healthy Chinese individuals, plasma corin levels ranged from 216 to 1663 pg/ml. The levels were higher in males than that in females (842 ± 283 vs. 569 ± 192 pg/ml, p<0.001). Soluble corin was stable in plasma samples. Plasma soluble corin levels vary depending on anticoagulants used. Samples containing heparin had significantly higher levels of corin than that in samples with EDTA or sodium citrate.
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影响因子：
158.5
作者：
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通讯作者：
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DOI：
10.1073/pnas.0407234102
发表时间：
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