Stimulation of superficial zone protein accumulation by hedgehog and Wnt signaling in surface zone bovine articular chondrocytes.
Stimulation of superficial zone protein accumulation by hedgehog and Wnt signaling in surface zone bovine articular chondrocytes.
复制标题
DOI：
10.1002/art.37768
复制
发表时间：
2013-02
影响因子：
--
通讯作者：
Reddi, A. Hari
中科院分区：
文献类型：
--
作者：
Iwakura, Takashi;Inui, Atsuyuki;Reddi, A. Hari
探讨刺猬蛋白和Wnt信号通路在关节软骨细胞表面区浅带蛋白（SZP）积累中的作用。从小腿膝关节软骨表面区分离软骨细胞，在无血清化学培养基中培养成单层软骨细胞。研究了SZP在培养基中积累对hedgehog蛋白（Sonic hedgehog； Shh和Indian hedgehog； Ihh）、Wnt蛋白（Wnt-3a、-5a和-11）、Wnt/β-catenin通路激动剂（糖原合成酶激酶3β； GSK-3β抑制剂）和Wnt/β-catenin通路拮抗剂的响应。免疫印迹法分析Wnt-3a和GSK-3β抑制剂治疗后β-连环蛋白的积累情况。Hedgehog蛋白（Shh和Ihh）刺激SZP的积累。Wnt-3a和GSK-3β抑制剂激活Wnt/β-catenin通路可抑制SZP的积累，而Wnt-5a和-11可以激活与β-catenin无关的Wnt通路，但对SZP的积累没有影响。另一方面，Wnt/β-catenin通路拮抗剂刺激SZP积累。此外，转化生长因子-β1与hedgehog蛋白或Wnt/β-catenin通路拮抗剂联合处理对SZP积累有加性作用。Sonic和印度刺猬信号通路对表面区关节软骨细胞的SZP积累有深远的影响。这些结果为更好地理解关节软骨的内稳态和维持提供了见解。
To investigate the roles of hedgehog and Wnt signaling pathways on accumulation of superficial zone protein (SZP) in surface zone articular chondrocytes. Chondrocytes were isolated from surface zone of articular cartilage from calf stifle joints and cultured as monolayers in serum-free chemically defined medium. Accumulation of SZP in the culture medium in response to hedgehog proteins (Sonic hedgehog; Shh and Indian hedgehog; Ihh), Wnt proteins (Wnt-3a, -5a and -11), agonists of Wnt/β-catenin pathway (glycogen synthase kinase-3β; GSK-3β inhibitors), and antagonists of Wnt/β-catenin pathway was investigated. Accumulation of β-catenin after treatment with Wnt-3a and GSK-3β inhibitor was also analyzed by immunoblots. Hedgehog proteins (Shh and Ihh) stimulated SZP accumulation. Activation of Wnt/β-catenin pathway by Wnt-3a and GSK-3β inhibitors led to inhibition of SZP accumulation, but Wnt-5a and -11 which can activate β-catenin-independent Wnt pathways had no influence on SZP accumulation. On the other hand, antagonists of Wnt/β-catenin pathway stimulated SZP accumulation. In addition, there were additive effects on SZP accumulation by combined treatment of transforming growth factor-β1 and hedgehog proteins or antagonists of Wnt/β-catenin pathway. Sonic and Indian hedgehog signaling pathway has a profound effect on SZP accumulation by surface zone articular chondrocytes. These results provide insights into a better understanding of articular cartilage homeostasis and maintenance.
登录
查看更多内容
影响因子：
7.8
作者：
Hinck, L;Nelson, W J;Papkoff, J
通讯作者：
Papkoff, J
影响因子：
--
作者：
Elsaid, Khaled A.;Jay, Gregory D.;Chichester, Clinton O.
通讯作者：
Chichester, Clinton O.
影响因子：
--
作者：
Neu, Corey P.;Khalafi, Afshin;Reddi, A. Hari
通讯作者：
Reddi, A. Hari
影响因子：
--
作者：
Chen, Xuesong;Macica, Carolyn M.;Nasiri, Ali;Broadus, Arthur E.
通讯作者：
Broadus, Arthur E.
影响因子：
64.5
作者：
Molenaar, M;vandeWetering, M;Clevers, H
通讯作者：
Clevers, H