Regulation of articular chondrocyte proliferation and differentiation by indian hedgehog and parathyroid hormone-related protein in mice.
Regulation of articular chondrocyte proliferation and differentiation by indian hedgehog and parathyroid hormone-related protein in mice.
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DOI：
10.1002/art.23985
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发表时间：
2008-12
影响因子：
--
通讯作者：
Broadus, Arthur E.
中科院分区：
文献类型：
--
作者：
Chen, Xuesong;Macica, Carolyn M.;Nasiri, Ali;Broadus, Arthur E.
骺生长区的软骨细胞受印度刺猬（Ihh）-甲状旁腺相关蛋白（PTHrP）轴的调节。在负重关节中，该生长区被次级骨化中心细分为不同的关节和生长软骨结构。在这里，我们探讨了细胞的起源，本地化，表达调控，和推定的功能Ihh和PTHrP在小鼠关节软骨。我们评估了Ihh和PTHrP的表达在等位基因PTHrP-lacZ敲入小鼠和几个版本的PTHrP无效小鼠。选择关节手术卸载检查PTHrP和Ihh的负荷诱导。胚胎生长区似乎作为PTHrP表达的增殖软骨细胞的来源，这些增殖软骨细胞填充形成关节软骨和生长板结构。在关节软骨中，这些细胞在中间区采取关节软骨细胞的形式。在PTHrP敲除小鼠中，矿化软骨细胞侵入发育中的关节软骨，但似乎被IHH驱动的表面软骨细胞增殖阻止矿化关节间隙。在生长和成年小鼠中，PTHrP在关节软骨细胞中的表达是负荷诱导的，并且卸载与PTHrP表达和关节软骨细胞分化的快速变化相关。我们的结论是PTHrP-Ihh轴参与关节软骨的维护。该系统的失调可能有助于关节炎的发病机制。
The chondrocytes of the epiphyseal growth zone are regulated by the Indian hedgehog (Ihh)-parathyroid hormone-related protein (PTHrP) axis. In weight-bearing joints, this growth zone comes to be subdivided by the secondary ossification center into distinct articular and growth cartilage structures. Here, we explored the cells of origin, localization, regulation of expression, and putative functions of Ihh and PTHrP in articular cartilage in the mouse. We assessed Ihh and PTHrP expression in an allelic PTHrP-lacZ knockin mouse and several versions of PTHrP-null mice. Selected joints were unloaded surgically to examine load-induction of PTHrP and Ihh. The embryonic growth zone appears to serve as the source of PTHrP-expressing proliferative chondrocytes that populate both the forming articular cartilage and growth plate structures. In articular cartilage, these cells take the form of articular chondrocytes in the mid-zone. In PTHrP-knockout mice, mineralizing chondrocytes encroach upon developing articular cartilage but appear to be prevented from mineralizing the joint space by Ihh-driven surface chondrocyte proliferation. In growing and adult mice, PTHrP expression in articular chondrocytes is load-induced, and unloading is associated with rapid changes in PTHrP expression and articular chondrocyte differentiation. We conclude that the PTHrP-Ihh axis participates in the maintenance of articular cartilage. Dysregulation of this system might contribute to the pathogenesis of arthritis.
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影响因子：
6.2
作者：
Chen, XS;Macica, CM;Broadus, AE
通讯作者：
Broadus, AE
DOI：
10.1302/0301-620x.69b5.3680348
发表时间：
1987-11-01
影响因子：
--
作者：
HOSHINO, A;WALLACE, WA
通讯作者：
WALLACE, WA
影响因子：
2.1
作者：
Pacifici, Maurizio;Koyama, Eiki;Iwamoto, Masahiro
通讯作者：
Iwamoto, Masahiro
DOI：
10.1111/j.1469-185x.1942.tb00440.x
发表时间：
1942-10-01
影响因子：
--
作者：
Haines, RW
通讯作者：
Haines, RW
影响因子：
--
作者：
Nolte, T;Kaufmann, W;Weber, E
通讯作者：
Weber, E