Oxidative stress inhibits axonal transport: implications for neurodegenerative diseases.
Oxidative stress inhibits axonal transport: implications for neurodegenerative diseases.
复制标题
DOI：
10.1186/1750-1326-7-29
复制
发表时间：
2012-06-18
影响因子：
15.1
通讯作者：
Banker G
中科院分区：
文献类型：
--
作者：
Fang C;Bourdette D;Banker G
小胶质细胞和其他炎症细胞释放的活性氧（ROS）可引起轴突变性。轴突运输的减少也被认为是轴突营养不良和神经变性的原因，但关于ROS对轴突运输的影响的实验数据很少。我们用活细胞成像研究过氧化氢对培养的大鼠海马神经元线粒体和高尔基体衍生囊泡轴突运输的影响。过氧化氢迅速抑制轴突运输，在任何可检测到的线粒体形态学变化或轴突变性的迹象前数小时。线粒体运输的影响更早，更严重的抑制比高尔基体衍生的囊泡的运输。顺行囊泡运输比逆行运输更容易受到过氧化物抑制。轴突运输部分恢复后，去除过氧化氢和局部应用过氧化氢抑制运输，表明这种影响不仅仅是神经细胞死亡的结果。ATP合成阻断剂叠氮化钠对轴突运输也有类似的影响，提示ATP耗竭可能是过氧化氢引起的运输抑制的原因。这些结果表明，轴突运输的抑制是暴露于ROS的早期后果，并可能导致随后的轴突变性。
Reactive oxygen species (ROS) released by microglia and other inflammatory cells can cause axonal degeneration. A reduction in axonal transport has also been implicated as a cause of axonal dystrophies and neurodegeneration, but there is a paucity of experimental data concerning the effects of ROS on axonal transport. We used live cell imaging to examine the effects of hydrogen peroxide on the axonal transport of mitochondria and Golgi-derived vesicles in cultured rat hippocampal neurons. Hydrogen peroxide rapidly inhibited axonal transport, hours before any detectable changes in mitochondrial morphology or signs of axonal degeneration. Mitochondrial transport was affected earlier and was more severely inhibited than the transport of Golgi-derived vesicles. Anterograde vesicle transport was more susceptible to peroxide inhibition than retrograde transport. Axonal transport partially recovered following removal of hydrogen peroxide and local application of hydrogen peroxide inhibited transport, suggesting that the effects were not simply a result of nerve cell death. Sodium azide, an ATP synthesis blocker, had similar effects on axonal transport, suggesting that ATP depletion may contribute to the transport inhibition due to hydrogen peroxide. These results indicate that inhibition of axonal transport is an early consequence of exposure to ROS and may contribute to subsequent axonal degeneration.
登录
查看更多内容
DOI：
10.1083/jcb.200601067
发表时间：
2006-05-22
期刊：
The Journal of cell biology
影响因子：
--
作者：
Glater EE;Megeath LJ;Stowers RS;Schwarz TL
通讯作者：
Schwarz TL
影响因子：
5.3
作者：
Her, Lu-Shiun;Goldstein, Lawrence S. B.
通讯作者：
Goldstein, Lawrence S. B.
影响因子：
64.8
作者：
BANKER, G;GOSLIN, K
通讯作者：
GOSLIN, K
DOI：
10.1007/978-3-642-00150-5_29
发表时间：
2010-01-01
期刊：
SIGNALING PATHWAYS IN LIVER DISEASES, SECOND EDITION
影响因子：
--
作者：
Fernandez-Checa, Jose C.;Garcia-Ruiz, Carmen
通讯作者：
Garcia-Ruiz, Carmen
影响因子：
14.8
作者：
Kaech, Stefanie;Banker, Gary
通讯作者：
Banker, Gary