A hydrogel spinal dural patch with potential anti-inflammatory, pain relieving and antibacterial effects.
A hydrogel spinal dural patch with potential anti-inflammatory, pain relieving and antibacterial effects.
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DOI：
10.1016/j.bioactmat.2022.01.043
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发表时间：
2022-08
影响因子：
18.9
通讯作者：
Zhao Y
中科院分区：
文献类型：
--
作者：
Li J;Tian J;Li C;Chen L;Zhao Y
硬脊膜缺损性脊髓型颈椎病是脊柱外科常见的并发症，需要缝合或使用封闭剂等修复。但手术缝合或修补封闭剂后，常出现低颅压症状、伤口感染、针孔漏或松动效应等住院时间延长。在潮湿环境中，需要稳定、耐压、高粘度的硬脊膜修补补片，无需缝合或密封剂。为此，提出了一种由钙离子交联的海藻酸盐和聚丙烯酰胺水凝胶基质与壳聚糖胶粘剂组成的生物活性贴片。该贴片在湿环境中的爆破压力大于790 mm·H2O时，具有促进缺陷闭合的能力和良好的密封性。此外，贴片的壳聚糖胶粘层在体外对细菌的生长有抑制作用，对术后感染有一定的意义。此外，在体内实验中，该贴剂还能显著降低术后早期胶质纤维酸性蛋白、IBA-1、基质金属结合蛋白、肿瘤坏死因子-α和环氧合酶-2的表达，发挥抗炎、镇痛和预防粘连的作用。因此，生物活性补片有望应用于脊髓硬脑膜修复，具有耐高压、促进创面闭合、抑制术后感染等良好性能。经0.25M钙离子二次处理后的水凝胶基质与壳聚糖粘合剂相结合，可用于湿法粘接和硬脑膜修复。其封闭效果满足硬脑膜缺损修复的基本需要，具有促进纤维细胞增殖、抗菌、抗炎、止痛、防止粘连等潜在功能。一种可直接按压的自粘性脊髓硬脑膜贴片。脊柱硬脑膜贴片在潮湿的环境中保持790 mm以上的H2O密封压力。一种具有潜在抗炎、止痛和抗菌特性的硬脊膜贴片。
CSFL caused by spinal dural defect is a common complication of spinal surgery, which need repair such as suture or sealants. However, low intracranial pressure symptoms, wound infection and prolonged hospital associated with pin-hole leakage or loose seal effect were often occurred after surgical suture or sealants repair. Stable, pressure resistance and high viscosity spinal dural repair patch in wet environment without suture or sealants was highly needed. Herein, a bioactive patch composed of alginate and polyacrylamide hydrogel matrix cross-linked by calcium ions, and chitosan adhesive was proposed. This fabricated patch exhibits the capabilities of promoting defect closure and good tight seal ability with the bursting pressure is more than 790 mm H2O in wet environment. In addition, the chitosan adhesive layer of the patch could inhibit the growth of bacterial in vitro, which is meaningful for the postoperative infection. Furthermore, the patch also significantly reduced the expression of GFAP, IBA-1, MBP, TNF-α, and COX-2 in early postoperative period in vivo study, exerting the effects of anti-inflammatory, analgesic and adhesion prevention. Thus, the bioactive patch expected to be applied in spinal dural repair with the good properties of withstanding high pressure, promoting defect closure and inhibiting postoperative infection. A hydrogel matrix after 0.25 M calcium ion secondary treatment, combined with a chitosan adhesive, can be used for wet bonding and dural repair. Its sealing effect meets the basic needs of dural defect repair, and has the potential functions of promoting fiber cell proliferation, antibacterial, anti-inflammatory, analgesic and preventing adhesion. A self-adhesive spinal dural patch that can be applied directly by pressing. A spinal dural patch maintains more than 790 mm H2O sealing pressure in a wet environment. A spinal dural patch with potential anti-inflammatory, analgesic and anti-bacterial properties.
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影响因子：
1.7
作者：
Shimada, Yoichi;Hongo, Michio;Itoi, Eiji
通讯作者：
Itoi, Eiji
DOI：
10.1007/s10856-021-06552-4
发表时间：
2021-07-23
期刊：
Journal of materials science. Materials in medicine
影响因子：
--
作者：
Kinaci A;van Thoor S;Redegeld S;Tooren M;van Doormaal TPC
通讯作者：
van Doormaal TPC
影响因子：
4.2
作者：
Iannotti, Christopher;Zhang, Y. Ping;Shields, Christopher B.
通讯作者：
Shields, Christopher B.
影响因子：
2
作者：
Bohoun, Christian A.;Goto, Takeo;Ohata, Kenji
通讯作者：
Ohata, Kenji
DOI：
10.1126/science.aah6362
发表时间：
2017-07-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Li J;Celiz AD;Yang J;Yang Q;Wamala I;Whyte W;Seo BR;Vasilyev NV;Vlassak JJ;Suo Z;Mooney DJ
通讯作者：
Mooney DJ