Signaling pathways in ascidian oocyte maturation: Effects of various inhibitors and activators on germinal vesicle breakdown
Signaling pathways in ascidian oocyte maturation: Effects of various inhibitors and activators on germinal vesicle breakdown
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海鞘卵母细胞成熟的信号通路：各种抑制剂和激活剂对生发囊泡破裂的影响
DOI：
10.1111/j.1440-169x.2005.00796.x
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发表时间：
2005
期刊：
影响因子：
4.6
通讯作者：
C. Lambert
中科院分区：
文献类型：
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作者：
C. Lambert
海鞘类是无脊椎动物的脊索动物，包括三个目；鞭毛纲是最复杂的。到目前为止，卵母细胞成熟(生发泡破裂)的开始只在一个单一的侏儒(Halocynthia Roretzi)中被研究过，在这种情况下，生发泡破裂(GVBD)是当卵母细胞接触海水(Sw)时开始的；对内部事件一无所知。这项研究有力地表明了蛋白质磷酸化在这一过程中的重要性。苍白赫德曼线虫(Puridae)的功能类似于H.roretzi；GVBD发生在西南部。线虫(Styelidae)卵母细胞不会自发地发生GVBD，但必须被激活。用pH 4Sw抑制Herdmania卵母细胞的GVBD，然后用Mastoparan(G蛋白激活剂)、A23187(钙离子载体)或二甲基苯并菲(酪氨酸激酶激活剂)激活。这需要成熟促进因子(MPF)的活性；细胞周期蛋白依赖的激酶抑制剂罗索维汀和洛莫辛具有抑制作用。它还需要去磷酸化，磷酸酶抑制剂维生素K3抑制GVBD的能力证明了这一点。酪氨酸激酶抑制剂Tyrphostin A23和Genistein以及磷脂酰肌醇-3-激酶抑制剂LY-294002也可抑制海星的血管性血友病。LY-294002在被马多巴或离子载体激活时有强烈的抑制作用，而在二甲基苯并菲激活时则无明显抑制作用。DMBA被假设为直接或间接地使磷酸酶磷酸化，绕过抑制而导致二次激活。
The Ascidiacea, the invertebrate chordates, includes three orders; the Stolidobranchia is the most complex. Until the present study, the onset of oocyte maturation (germinal vesicle breakdown) had been investigated in only a single pyurid (Halocynthia roretzi), in which germinal vesicle breakdown (GVBD) begins when the oocyte contacts seawater (SW); nothing was known about internal events. This study strongly suggests the importance of protein phosphorylation in this process. Herdmania pallida (Pyuridae) functions like H. roretzi; GVBD occurs in SW. Oocytes of Cnemidocarpa irene (Styelidae) do not spontaneously undergo GVBD in SW but must be activated. Herdmania oocytes are inhibited from GVBD by pH 4 SW and subsequently activated by mastoparan (G‐protein activator), A23187 (Ca2+ ionophore) or dimethylbenzanthracene (tyrosine kinase activator). This requires maturation promoting factor (MPF) activity; cyclin‐dependent kinase inhibitors roscovitine and olomoucine are inhibitory. It also entails dephosphorylation as demonstrated by the ability of the phosphatase inhibitor vitamin K3 to inhibit GVBD. GVBD is also inhibited by the tyrosine kinase inhibitors tyrphostin A23 and genistein, and LY‐294002, a phosphatidylinositol‐3‐kinase inhibitor previously shown to inhibit starfish GVBD. LY‐294002 inhibits strongly when activation is by mastoparan or ionophore but not when activated by dimethylbenzanthracene (DMBA). The DMBA is hypothesized to phosphorylate a phosphatase directly or indirectly causing secondary activation, bypassing inhibition.
生长因子和卵巢功能：IGF-I 范式。
影响因子：
--
作者：
Adashi,EY
通讯作者：
Adashi,EY