Fast, single-molecule localization that achieves theoretically minimum uncertainty.
Fast, single-molecule localization that achieves theoretically minimum uncertainty.
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DOI:
10.1038/nmeth.1449
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发表时间:
2010-05
期刊:
影响因子:
48
通讯作者:
Lidke, Keith A.
中科院分区:
文献类型:
--
作者:
Smith, Carlas S.;Joseph, Nikolai;Rieger, Bernd;Lidke, Keith A.
We describe an iterative algorithm that converges to the maximum likelihood estimate of the position and intensity of a single fluorophore. Our technique efficiently computes and achieves the Cramér-Rao lower bound, an essential tool for parameter estimation. An implementation of the algorithm on graphics processing unit hardware achieved more than 105combined fits and Cramér-Rao lower bound calculations per second, enabling real-time data analysis for super-resolution imaging and other applications.
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