Protective effect of estrogen in endothelin-induced middle cerebral artery occlusion in female rats.
Protective effect of estrogen in endothelin-induced middle cerebral artery occlusion in female rats.
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DOI：
10.1016/j.neulet.2008.09.006
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发表时间：
2008-11-14
影响因子：
2.5
通讯作者：
Schreihofer DA
中科院分区：
文献类型：
--
作者：
Glendenning ML;Lovekamp-Swan T;Schreihofer DA
雌激素在脑损伤（包括局灶性脑缺血）动物模型中是一种强大的内源性和外源性神经保护剂。虽然这种保护作用已在几种不同的治疗和损伤范例中得到证实，但在脑实质内注射内皮素-1诱导的局灶性脑缺血中尚未得到证实，该模型与其他实验性局灶性缺血模型相比具有许多优势。将生殖成熟的雌性Sprague-Dawley大鼠切除卵巢，并分为安慰剂组和雌二醇治疗组。两周后，氟烷麻醉的大鼠通过脑实质间立体定向注射大脑中动脉附近的强效血管收缩剂内皮素1（180 pmole/2 µl）进行大脑中动脉（MCA）闭塞。激光多普勒血流仪（LDF）显示两组脑血流量减少相似。在中风诱导之前和两天后对动物进行行为学评价，并评价梗死面积。与其他模型一致，雌激素治疗显著降低了TTC和Fluoro-Jade染色评估的梗死面积以及与卒中相关的行为缺陷。在两组中，卒中大小与LDF显著相关，表明颅骨灌注测量可以提高该模型的成功率。
Estrogen is a powerful endogenous and exogenous neuroprotective agent in animal models of brain injury, including focal cerebral ischemia. Although this protection has been demonstrated in several different treatment and injury paradigms, it has not been demonstrated in focal cerebral ischemia induced by intraparenchymal endothelin-1 injection, a model with many advantages over other models of experimental focal ischemia. Reproductively mature female Sprague-Dawley rats were ovariectomized and divided into placebo and estradiol-treated groups. Two weeks later, halothane-anesthetized rats underwent middle cerebral artery (MCA) occlusion by interparenchymal stereotactic injection of the potent vasoconstrictor endothelin 1 (180 pmoles/2 µl) near the middle cerebral artery. Laser-Doppler flowmetry (LDF) revealed similar reductions in cerebral blood flow in both groups. Animals were behaviorally evaluated before, and two days after, stroke induction, and infarct size was evaluated. In agreement with other models, estrogen treatment significantly reduced infarct size evaluated by both TTC and Fluoro-Jade staining and behavioral deficits associated with stroke. Stroke size was significantly correlated with LDF in both groups, suggesting that cranial perfusion measures can enhance success in this model.
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影响因子：
8.3
作者：
Matsuo, Y;Mihara, S;Fujimoto, M
通讯作者：
Fujimoto, M
影响因子：
3
作者：
Baldauf, Kathrin;Henrich-Noack, Petra;Reymann, Klaus G.
通讯作者：
Reymann, Klaus G.
影响因子：
2.9
作者：
O'Neill, MJ;Hicks, CA;Boot, JR
通讯作者：
Boot, JR
EGF/bFGF治疗对一过性局灶性缺血后增殖、早期神经发生和梗死体积的影响
影响因子：
2.9
作者：
Baldauf, K;Reymann, KG
通讯作者：
Reymann, KG
DOI：
10.1152/ajpheart.00809.2006
发表时间：
2007-01-01
影响因子：
4.8
作者：
Ba, Zheng F.;Lu, Ailing;Chaudry, Irshad H.
通讯作者：
Chaudry, Irshad H.