Streptozotocin treatment at birth induces a parallel depletion of thyrotropin-releasing hormone and insulin in the rat pancreas during development.-猫眼课题宝

出生时链脲佐菌素治疗会导致大鼠胰腺在发育过程中同时消耗促甲状腺素释放激素和胰岛素。

DOI：

发表时间：

1984

期刊：

Endocrinology

影响因子：

4.8

通讯作者：

中科院分区：

医学2区

文献类型：

作者：

S. Aratan;B. Wolf;B. Portha;D. Bailbé;P. Czernichow

在生命早期，已在大鼠胰腺中检测到 TRH 浓度升高。本研究的目的是使用出生时注射的链脲佐菌素 (STZ) 进一步研究该肽在胰腺中的细胞位置。对注射 STZ 的大鼠从出生到 35 天的不同年龄测量胰腺 TRH 和胰岛素含量，并将结果与注射媒介物的对照组进行比较。从第 1 天到第 5 天观察到短暂的高血糖状态（最大值 2.9 +/- 0.31 g/L）。此后，尽管 STZ 大鼠血糖轻微升高，但不再出现糖尿。 TRH 和胰岛素含量从第 1 至 5 天和第 5 至 35 天遵循两种不同的模式。在第一个时期，观察到两种物质的急性消耗，观察到的较低值分别达到 TRH 和胰岛素对照值的 2.7% 和 9%。第 2 天的 TRH 激增减弱。在第二阶段，胰岛素含量增加至对照组的 42%。相反，没有观察到TRH的恢复；第 35 天时，TRH 含量为对照的 9%。这些结果表明 TRH 位于 STZ 敏感细胞中，与最近的免疫组织化学数据一致。 TRH 恢复能力受损仍然无法解释，似乎表明胰岛素和 TRH 的生物发生存在差异。

Elevated concentrations of TRH have been detected in the rat pancreas during the early days of life. The purpose of this study was to investigate further the cellular location of this peptide in the pancreas using streptozotocin (STZ) injected at birth. Pancreatic TRH and insulin contents were measured at different ages from birth until 35 days in rats injected with STZ and results compared with controls injected with the vehicle. A transitory hyperglycemic state was observed from day 1 to day 5 (maximum value 2.9 +/- 0.31 g/liter). After this period, although slightly hyperglycemic, STZ rats were not glucosuric . TRH and insulin contents followed two distinct patterns from days 1 to 5 and days 5 to 35. During the first period, an acute depletion of both substances was observed, the lower value observed reaching 2.7% and 9% of control values, respectively, for TRH and insulin. The TRH surge at day 2 was blunted. During the second period, insulin content increased to reach 42% of controls. On the contrary, recovery of TRH was not observed; TRH content was 9% of control at day 35. These results indicate that TRH is located in STZ-sensitive cells, in agreement with recent immunohistochemical data. The impaired capacity for TRH recovery remained unexplained and seems to indicate a difference in the biogenesis of insulin and TRH.

Ontogenetic patterns of thyrotropin-releasing hormone-like material in rat hypothalamus, pancreas, and retina: selective effect of light deprivation.