Moth sex pheromone receptors and deceitful parapheromones.
Moth sex pheromone receptors and deceitful parapheromones.
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DOI：
10.1371/journal.pone.0041653
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Leal WS
中科院分区：
文献类型：
--
作者：
Xu P;Garczynski SF;Atungulu E;Syed Z;Choo YM;Vidal DM;Zitelli CH;Leal WS
这种昆虫的嗅觉系统是如此的有选择性，例如，雄蛾可以区分雌性产生的性信息素和结构变化最小的化合物。然而，对于这种“锁和钥匙”的严格选择性有一个例外。甲酸酯类似物可用作化学稳定性较低的长链醛信息素的替代物，因为雄性蛾对这些准信息素有生理和行为反应。然而，迄今为止，甲酸类似物如何与环糊精敏感的气味受体（OR）相互作用仍然是未知的。用单感器记录法研究了神经元对化学信息素的反应。利用简并引物克隆了气味受体（OR），并用非洲爪蟾卵母细胞表达系统进行了测试。采用气相色谱法和气相色谱-质谱联用技术对样品的质量、相对含量和纯度进行了评价。我们发现嗅觉受体神经元（ORN）安置在触角上的雄性脐橙幼虫的触角，同样的反应性信息素的主要成分，（11 Z，13 Z）-十六碳二烯醛（Z11 Z 13 - 16 Ald），和它的甲酸类似物，（9 Z，11 Z）-十四碳烯-1-基甲酸酯（Z9 Z 11 - 14 OFor）。我们克隆了一个气味受体辅助受体（Orco）和一个对气味敏感的OR，其中一个（AtraOR 1）在雄性触角中特异性表达。表达AtraOR 1·AtraOR的卵母细胞主要对Z11 Z13 - 16 Ald有反应，对性信息素的另一种组分（11 Z，13 Z）-十六碳二烯-1-醇有中等敏感性。令人惊讶的是，这种受体是更敏感的有关甲酸比天然性信息素。来自烟芽夜蛾的信息素受体HR 13（= HvirOR 13）表现出类似的特征，甲酸酯类似物引起的反应比天然性信息素（11 Z）-十六烯醛引起的反应更强，从而表明这可能是蛾类信息素受体的共同特征。
The insect's olfactory system is so selective that male moths, for example, can discriminate female-produced sex pheromones from compounds with minimal structural modifications. Yet, there is an exception for this “lock-and-key” tight selectivity. Formate analogs can be used as replacement for less chemically stable, long-chain aldehyde pheromones, because male moths respond physiologically and behaviorally to these parapheromones. However, it remained hitherto unknown how formate analogs interact with aldehyde-sensitive odorant receptors (ORs). Neuronal responses to semiochemicals were investigated with single sensillum recordings. Odorant receptors (ORs) were cloned using degenerate primers, and tested with the Xenopus oocyte expression system. Quality, relative quantity, and purity of samples were evaluated by gas chromatography and gas chromatography-mass spectrometry. We identified olfactory receptor neurons (ORNs) housed in trichoid sensilla on the antennae of male navel orangeworm that responded equally to the main constituent of the sex pheromone, (11Z,13Z)-hexadecadienal (Z11Z13-16Ald), and its formate analog, (9Z,11Z)-tetradecen-1-yl formate (Z9Z11-14OFor). We cloned an odorant receptor co-receptor (Orco) and aldehyde-sensitive ORs from the navel orangeworm, one of which (AtraOR1) was expressed specifically in male antennae. AtraOR1•AtraOrco-expressing oocytes responded mainly to Z11Z13-16Ald, with moderate sensitivity to another component of the sex pheromone, (11Z,13Z)-hexadecadien-1-ol. Surprisingly, this receptor was more sensitive to the related formate than to the natural sex pheromone. A pheromone receptor from Heliothis virescens, HR13 ( = HvirOR13) showed a similar profile, with stronger responses elicited by a formate analog than to the natural sex pheromone, (11Z)-hexadecenal thus suggesting this might be a common feature of moth pheromone receptors.
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DOI：
10.1073/pnas.0501447102
发表时间：
2005-04-12
影响因子：
11.1
作者：
Leal, WS;Chen, AM;Tsuruda, JM
通讯作者：
Tsuruda, JM
影响因子：
2.2
作者：
Kuenen, L. P. S.;McElfresh, J. Steven;Millar, Jocelyn G.
通讯作者：
Millar, Jocelyn G.
影响因子：
2.3
作者：
TODD, JL;MILLAR, JG;BAKER, TC
通讯作者：
BAKER, TC
影响因子：
3.7
作者：
Leal WS;Ishida Y;Pelletier J;Xu W;Rayo J;Xu X;Ames JB
通讯作者：
Ames JB
影响因子：
1
作者：
KEIL, TA
通讯作者：
KEIL, TA