Comparative performance of PCR using DNA extracted from dried blood spots and whole blood samples for malaria diagnosis: a meta-analysis.
Comparative performance of PCR using DNA extracted from dried blood spots and whole blood samples for malaria diagnosis: a meta-analysis.
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使用干血斑和全血样本提取的DNA进行PCR诊断疟疾的比较性能：荟萃分析。
DOI：
10.1038/s41598-021-83977-5
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发表时间：
2021-03-01
影响因子：
4.6
通讯作者：
Kotepui M
中科院分区：
文献类型：
--
作者：
Mahittikorn A;Masangkay FR;Kotepui KU;De Jesus Milanez G;Kotepui M
利用从干血斑中提取的脱氧核糖核酸(DNA)进行聚合酶链式反应(PCR)为大规模流行病学研究提供了一种快速、廉价和方便的方法。本研究比较了从DBS中提取的DNA和从全血中提取的DNA在检测疟疾寄生虫方面的性能。从ISI Web of Science、Scope us和PubMed数据库中获得了评估从DBS和全血中提取的DNA用于检测疟疾寄生虫的PCR诊断性能的初步研究。优势比(OR)和95%可信区间(CI)是使用Review Manager版本5.3在森林地块中绘制的。采用随机效应Meta分析进行统计分析。使用I2统计量评估数据的异质性。在从数据库中检索到的904项研究中，有7项纳入了这项研究。合并Meta分析显示，两种方法检测疟疾原虫的效果差异无统计学意义(OR0.85；95%CI0.62~1.16；I2 = 78%)。但亚群分析显示，与从全血中提取的DNA相比，从DBS提取的DNA检测间日疟原虫的准确性较低(OR = 为0.85；95%CI为0.77~0.94)。总之，从DBS中提取的DNA与从全血中提取的DNA在检测间日疟原虫的方法上存在显著差异。因此，流行区间日疟原虫的鉴定和检测应谨慎使用从DBS获得的DNA进行聚合酶链式反应，这可能导致阴性结果。需要进行进一步的研究，以调查利用DBS检测间日疟原虫和其他疟疾寄生虫的聚合酶链式反应的性能，以便为疟疾的研究和常规监测提供数据，特别是在为根除该疾病作出新的努力的情况下。
Polymerase chain reaction (PCR) using deoxyribonucleic acid (DNA) extracted from dried blood spots (DBS) provides a fast, inexpensive, and convenient method for large-scale epidemiological studies. This study compared the performance of PCR between DNA extracted from DBS and DNA obtained from whole blood for detecting malarial parasites. Primary studies assessing the diagnostic performance of PCR using DNA extracted from DBS and whole blood for detecting malarial parasites were obtained from the ISI Web of Science, Scopus, and PubMed databases. Odds ratios (ORs) and 95% confidence intervals (CIs) were plotted in forest plots using Review Manager version 5.3. Statistical analysis was performed via random-effects meta-analysis. Data heterogeneity was assessed using the I2 statistic. Of the 904 studies retrieved from the databases, seven were included in this study. The pooled meta-analysis demonstrated no significant difference in the comparative performance of PCR for detecting malaria parasites between DNA extracted from DBS and that extracted from whole blood (OR 0.85; 95% CI 0.62–1.16; I2 = 78%). However, subgroup analysis demonstrated that PCR using DNA extracted from DBS was less accurate in detecting Plasmodium vivax than that using DNA extracted from whole blood (OR = 0.85; 95% CI 0.77–0.94). In conclusion, a significant difference in detecting P. vivax was observed between PCR using DNA extracted from DBS and that using DNA extracted from whole blood. Therefore, P. vivax in endemic areas should be identified and detected with care with PCR using DNA obtained from DBS which potentially leads to a negative result. Further studies are required to investigate the performance of PCR using DBS for detecting P. vivax and other malarial parasites to provide data in research and routine surveillance of malaria, especially with renewed efforts towards the eradication of the disease.
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影响因子：
4.6
作者：
Kotepui, Manas;Kotepui, Kwuntida Uthaisar;Masangkay, Frederick Ramirez
通讯作者：
Masangkay, Frederick Ramirez
DOI：
10.1016/s0035-9203(99)90177-3
发表时间：
1999-01-01
影响因子：
2.2
作者：
Färnert, A;Arez, AP;do Rosário, V
通讯作者：
do Rosário, V
影响因子：
3
作者：
Zainabadi K;Adams M;Han ZY;Lwin HW;Han KT;Ouattara A;Thura S;Plowe CV;Nyunt MM
通讯作者：
Nyunt MM
在赤道几内亚的代表性样本中检测三种诊断方法（显微镜，RDT和PCR）的三种诊断方法（显微镜，RDT和PCR）的比较。
影响因子：
3
作者：
Berzosa P;de Lucio A;Romay-Barja M;Herrador Z;González V;García L;Fernández-Martínez A;Santana-Morales M;Ncogo P;Valladares B;Riloha M;Benito A
通讯作者：
Benito A
DOI：
10.4269/ajtmh.1999.60.183
发表时间：
1999-02-01
影响因子：
3.3
作者：
Rubio, JM;Benito, A;Alvar, J
通讯作者：
Alvar, J