Microfabricated ultrarapid desalting device for nanoelectrospray ionization mass spectrometry.
Microfabricated ultrarapid desalting device for nanoelectrospray ionization mass spectrometry.
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DOI：
10.1021/ac5040083
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发表时间：
2015-01-06
影响因子：
7.4
通讯作者：
Fedorov, Andrei G.
中科院分区：
文献类型：
--
作者：
Tibavinsky, Ivan A.;Kottke, Peter A.;Fedorov, Andrei G.
除盐是生物样品电喷雾电离质谱 (ESI-MS) 分析的先决条件。时间分辨测量需要快速脱盐和与电喷雾尖端的小体积连接。我们开发了一种满足这两个要求的微型脱盐装置，从而为复杂生物液体样本的瞬态 ESI-MS 测量提供了基础技术。在微制造装置中，样品在通道中流动，该通道通过整体集成的纳米多孔氧化铝膜与更高流速的无盐反溶液分开，该膜可以支持流道之间超过 600 kPa 的压差。利用盐与典型 ESI-MS 目标生物分析物（例如肽和蛋白质）之间扩散率的巨大差异来去除盐。我们证明了能够在~1秒内从样品溶液中去除95%的盐，同时保留足够高浓度的相对低分子量的蛋白质细胞色素-c，用于ESI-MS检测。
Salt removal is a prerequisite for electrospray ionization mass spectrometry (ESI-MS) analysis of biological samples. Rapid desalting and a low volume connection to an electrospray tip are required for time-resolved measurements. We have developed a microfabricated desalting device that meets both requirements, thus providing the foundational technology piece for transient ESI-MS measurements of complex biological liquid specimens. In the microfabricated device, the sample flows in a channel separated from a higher flow rate, salt-free counter solution by a monolithically integrated nanoporous alumina membrane, which can support pressure differences between the flow channels of over 600 kPa. Salt is removed by exploiting the large difference in diffusivities between salts and the typical ESI-MS target bioanalytes, e.g., peptides and proteins. We demonstrate the capability to remove 95% of salt from a sample solution in ∼1 s while retaining sufficiently high concentration of a relatively low molecular weight protein, cytochrome-c, for ESI-MS detection.
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影响因子：
4.2
作者：
Roach, Patrick J.;Laskin, Julia;Laskin, Alexander
通讯作者：
Laskin, Alexander
影响因子：
7.4
作者：
Wilm, M;Mann, M
通讯作者：
Mann, M
一种简便的微透析接口，用于通过纳米电喷雾电离质谱法在线脱盐和鉴定蛋白质
影响因子：
2
作者：
Sun, Liangliang;Duan, Jicheng;Zhang, Yukui
通讯作者：
Zhang, Yukui
影响因子：
7.4
作者：
Wilson, DJ;Konermann, L
通讯作者：
Konermann, L
影响因子：
2.3
作者：
Jakubowski, JA;Hatcher, NG;Sweedler, JV
通讯作者：
Sweedler, JV