Molecular cloning and mRNA expression analysis of ornithine decarboxylase antizyme 2 in ovarian follicles of the Sichuan white goose (Anser cygnoides).
Molecular cloning and mRNA expression analysis of ornithine decarboxylase antizyme 2 in ovarian follicles of the Sichuan white goose (Anser cygnoides).
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四川白鹅卵泡中鸟氨酸脱羧酶抗酶2的分子克隆及mRNA表达分析
DOI：
10.1016/j.gene.2014.05.022
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发表时间：
2014-07
期刊：
影响因子：
3.5
通讯作者：
Bai, Lin
中科院分区：
文献类型：
--
作者：
Kang, Bo;Jiang, Dongmei;Ma, Rong;Bai, Lin
鸟氨酸脱羧酶抗酶2(OAZ2)基因是抗酶基因家族的一员。Antizyme在多种细胞途径中发挥关键作用，包括多胺合成代谢和细胞凋亡。OAZ2在鹅卵泡中的分子结构和表达谱尚未见报道。本研究克隆了四川白鹅(Anser Cygnoids)OAZ2的基因序列，并对其进行了系统发育和结构分析。采用实时荧光定量聚合酶链式反应技术检测OAZ2mRNA在鹅卵泡中的表达。序列分析表明，该756bpOAZ2序列含有两个重叠的开放阅读框(ORF)。ORF1全长99个碱基对，编码32个氨基酸的多肽。ORF2全长477bp，编码158个氨基酸的多肽。启动ORF2翻译的移码位点位于OAZ2的第97位核苷酸。OAZ2mRNA在分级卵泡中的表达分析表明，在SWF和F2卵泡期OAZ2mRNA的表达水平高于卵巢间质(P<；P<0.05)。卵巢间质中OAZ2的表达水平最低。这些结果表明，高度保守的移码区在维持OAZ的功能中起着重要作用。此外，在SWF期OAZ2mRNA的表达水平显著升高，表明OAZ2可能参与了分级卵泡的招募。我们的结果还表明，OAZ2可能增强了OAZ1在卵泡发育中的作用。
The ornithine decarboxylase antizyme 2 (OAZ2) gene is a member of the antizyme gene family. Antizymes play pivotal roles in various cellular pathways, including polyamine anabolism and apoptosis. The molecular structure and expression profile of theOAZ2in goose ovarian follicles have not been reported. In this study, theOAZ2cDNA sequence of the Sichuan white goose was cloned (Anser cygnoides), and phylogenetic and structural analyses of theOAZ2were performed. The expression profiling ofOAZ2mRNA in goose ovarian follicles was examined using quantitative real-time PCR. The sequence analysis showed that the 756 bpOAZ2sequence contained two overlapping open reading frames (ORF). ORF1 was 99 bp in length, and encoded a 32 aa polypeptide. ORF2 was 477 bp in length, and encoded a 158 aa polypeptide. The frameshift site that initiates the translation of ORF2 was located at nucleotide position 97 in theOAZ2. The analysis ofOAZ2mRNA expression in hierarchical follicles showed that the level ofOAZ2mRNA was higher in the SWF and F2 follicular stages than that in the ovarian stroma (P< 0.05). The lowest level ofOAZ2expression was detected in the ovarian stroma. These results suggest that the highly conserved frameshift region plays an important role in sustaining the function ofOAZs. Furthermore, the significantly higher level ofOAZ2mRNA in the SWF stage indicates thatOAZ2may be involved in recruiting hierarchical follicles. Our results also suggest thatOAZ2may augment the effects ofOAZ1in follicle development.
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DOI：
10.1007/978-1-61779-034-8_1
发表时间：
2011
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Pegg, Anthony E;Casero, Robert A Jr
通讯作者：
Casero, Robert A Jr
影响因子：
4.4
作者：
Kang, B.;Guo, J. R.;Dong, C. Y.
通讯作者：
Dong, C. Y.
OAZ-T/OAZ3对于将精子尾巴与鼠标头部的刚性连接至关重要。
影响因子：
4.5
作者：
Tokuhiro K;Isotani A;Yokota S;Yano Y;Oshio S;Hirose M;Wada M;Fujita K;Ogawa Y;Okabe M;Nishimune Y;Tanaka H
通讯作者：
Tanaka H
DOI：
10.1074/jbc.274.37.26425
发表时间：
1999-09
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
C. Zhu;D. Wayne Lang;P. Coffino
通讯作者：
C. Zhu;D. Wayne Lang;P. Coffino
DOI：
--
发表时间：
2008
期刊：
Journal of Medical Molecular Biology
影响因子：
--
作者：
M. Wenli
通讯作者：
M. Wenli