Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes.
Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes.
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DOI：
10.1016/j.isci.2022.104797
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发表时间：
2022-09-16
期刊：
影响因子：
5.8
通讯作者：
McConnell, Gail
中科院分区：
文献类型：
--
作者：
Battistella, Eliana;Schniete, Jan;Wesencraft, Katrina;Quintana, Juan F.;McConnell, Gail
大的生物组织标本，如小鼠大脑的超厚切片的快速成像不能很容易地用标准显微镜进行。光学介观提供了一种解决方案，但到目前为止，成像速度太慢，无法用于常规用途。我们已经开发了两种不同的照明光片介观与Mesolens，我们证明了它们的使用在高速光学介观成像的大型组织标本。第一种光片方法使用高斯光学，并且易于实现。它提供了出色的横向分辨率和高速成像，但轴向分辨率很差。第二个光片是一个更复杂的艾里光片，提供亚细胞的分辨率在三维空间，是可比的质量点扫描共聚焦介观，但照明标本的光片方法减少了14倍的成像时间。这为中尺度的高含量、高通量光学生物成像创造了新的可能性。使用Airy和Gaussian光束的Mesolens光片介观成像在40 mm 3的成像体积内实现3D亚细胞分辨率成像时间比共焦介观成像缩短约14倍细胞生物学;光学成像;光学
Rapid imaging of large biological tissue specimens such as ultrathick sections of mouse brain cannot easily be performed with a standard microscope. Optical mesoscopy offers a solution, but thus far imaging has been too slow to be useful for routine use. We have developed two different illuminators for light-sheet mesoscopy with the Mesolens and we demonstrate their use in high-speed optical mesoscale imaging of large tissue specimens. The first light-sheet approach uses Gaussian optics and is straightforward to implement. It provides excellent lateral resolution and high-speed imaging, but the axial resolution is poor. The second light-sheet is a more complex Airy light-sheet that provides sub-cellular resolution in three dimensions that is comparable in quality to point-scanning confocal mesoscopy, but the light-sheet method of illuminating the specimen reduces the imaging time by a factor of 14. This creates new possibilities for high-content, higher-throughput optical bioimaging at the mesoscale. Light-sheet mesoscopy with the Mesolens using Airy and Gaussian beams Sub-cellular resolution in 3D within an imaging volume of 40 mm3 Approximately 14-fold reduction in imaging time compared to confocal mesoscopy Cell biology; Optical imaging; Optics
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影响因子：
48
作者：
Demas J;Manley J;Tejera F;Barber K;Kim H;Traub FM;Chen B;Vaziri A
通讯作者：
Vaziri A
影响因子：
16.6
作者：
Schmid, Benjamin;Shah, Gopi;Scherf, Nico;Weber, Michael;Thierbach, Konstantin;Campos, Citlali Perez;Roeder, Ingo;Aanstad, Pia;Huisken, Jan
通讯作者：
Huisken, Jan
影响因子：
4.6
作者：
Schniete J;Franssen A;Dempster J;Bushell TJ;Amos WB;McConnell G
通讯作者：
McConnell G
DOI：
10.1242/dev.116970
发表时间：
2015-03-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Bassi A;Schmid B;Huisken J
通讯作者：
Huisken J
DOI：
10.1016/b978-0-12-374920-8.00203-4
发表时间：
2011-01-01
期刊：
COMPREHENSIVE BIOPHYSICS, VOL 2: BIOPHYSICAL TECHNIQUES FOR CHARACTERIZATION OF CELLS
影响因子：
--
作者：
Amos, B.;McConnell, G.;Wilson, T.
通讯作者：
Wilson, T.