Neutrophil chemoattractant 2 beta regulates expression of the Reg gene in injured gastric mucosa in rats.
Neutrophil chemoattractant 2 beta regulates expression of the Reg gene in injured gastric mucosa in rats.
复制标题
中性粒细胞趋化剂 2β 调节大鼠受损胃粘膜中 Reg 基因的表达。
作者：
H. Kazumori;S. Ishihara;E. Hoshino;K. Kawashima;N. Moriyama;H. Suetsugu;H. Sato;K. Adachi;R. Fukuda;M. Watanabe;S. Takasawa;H. Okamoto;H. Fukui;T. Chiba;Y. Kinoshita
背景与目标
再生（Reg）蛋白对胃粘膜细胞具有营养作用。我们已经表明，Reg基因的表达增加肠嗜铬样（ECL）细胞在周围的粘膜糜烂损伤的胃粘膜愈合。本研究旨在探讨胃粘膜损伤愈合过程中Reg表达的刺激因素。
方法
在诱导大鼠胃粘膜损伤后，研究了各种促炎细胞因子和Reg基因表达的时程变化。使用逆流淘洗富集的大鼠ECL细胞在体外研究促炎细胞因子对Reg基因表达和Reg蛋白产生的直接影响。通过逆转录聚合酶链反应研究ECL细胞中CXC受体2（CXCR-2）的表达。还研究了用尼古丁诱导的中性粒细胞趋化因子（CINC-2 β）的中和抗体处理的大鼠中的Reg基因表达。
结果
在愈合过程中，几种促炎细胞因子和Reg的基因表达显著增强。在促炎性细胞因子中，CINC-2 β是其中增强的表达先于Reg基因表达增加的唯一细胞因子。在用CINC-2 β中和抗体处理的大鼠中，Reg基因表达的增加被显著抑制。当ECL细胞与这些促炎细胞因子孵育时，CINC-2 β剂量依赖性地增加ECL细胞中的Reg信使RNA和Reg蛋白。在分离的ECL细胞中鉴定了CXCR-2。
结论
CINC-2 β在受损胃粘膜中表达，通过CXCR-2刺激ECL细胞中Reg蛋白的产生，并可能参与加速受损胃粘膜的愈合。
BACKGROUND & AIMS
Regenerating (Reg) protein has a trophic effect on gastric mucosal cells. We have shown that Reg gene expression is increased in enterochromaffin-like (ECL) cells during the healing of damaged gastric mucosa around mucosal erosion. This study was designed to explore the stimulants of Reg expression during the healing of gastric mucosal damage.
METHODS
Time course changes of the expression of genes for various proinflammatory cytokines and Reg were investigated after induction of gastric mucosal lesions in rats. The direct effect of proinflammatory cytokines on Reg gene expression and Reg protein production were investigated in vitro using counterflow elutriation-enriched rat ECL cells. CXC receptor 2 (CXCR-2) expression was investigated in ECL cells by reverse-transcription polymerase chain reaction. Reg gene expression was also investigated in rats treated by the neutralizing antibody of cytokine-induced neutrophil chemoattractant (CINC-2 beta).
RESULTS
During healing, the gene expression of several proinflammatory cytokines and Reg was markedly augmented. Among the proinflammatory cytokines, CINC-2 beta is the only cytokine in which augmented expression preceded the increase of Reg gene expression. In rats treated with CINC-2 beta neutralizing antibody, the augmentation of Reg gene expression was significantly inhibited. When ECL cells were incubated with these proinflammatory cytokines, CINC-2 beta dose-dependently increased Reg messenger RNA and Reg protein in ECL cells. CXCR-2 was identified in isolated ECL cells.
CONCLUSIONS
CINC-2 beta, expressed in damaged gastric mucosa, stimulates the production of Reg protein in ECL cells via CXCR-2 and may be involved in the accelerated healing of injured gastric mucosa.
转化生长因子α在非洲啮齿动物乳腺瘤肠嗜铬样细胞肿瘤自主性中的作用。
影响因子：
29.4
作者：
Tang,LH;Modlin,IM;Lawton,GP;Kidd,M;Chinery,R
通讯作者：
Chinery,R
大鼠肠嗜铬样细胞上的生长抑素受体亚型。
影响因子：
29.4
作者：
Prinz,C;Sachs,G;Walsh,JH;Coy,DH;Wu,SV
通讯作者：
Wu,SV