High throughput small RNA and transcriptome sequencing reveal capacitation-related microRNAs and mRNA in boar sperm.
High throughput small RNA and transcriptome sequencing reveal capacitation-related microRNAs and mRNA in boar sperm.
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高通量小 RNA 和转录组测序揭示了公猪精子中与获能相关的 microRNA 和 mRNA。
DOI：
10.1186/s12864-018-5132-9
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发表时间：
2018-10-11
期刊：
影响因子：
4.4
通讯作者：
Zeng CJ
中科院分区：
文献类型：
--
作者：
Li Y;Li RH;Ran MX;Zhang Y;Liang K;Ren YN;He WC;Zhang M;Zhou GB;Qazi IH;Zeng CJ
获能是卵母细胞受精的先决条件，是一个复杂的过程，涉及精子的一系列结构和功能变化，例如膜修饰、酶活性调节和蛋白质磷酸化。为了穿透卵母细胞并使其受精，哺乳动物精子必须经历获能。然而，精子获能的过程仍然知之甚少，需要进一步阐明。在当前的研究中，通过高通量测序，我们鉴定并探索了参与公猪精子获能的差异表达的 microRNA (miRNA) 和 mRNA。我们总共鉴定了 5342 个 mRNA 和 204 个 miRNA，它们在新鲜和获能公猪精子中存在差异表达。其中，12 个 miRNA（8 个已知的和 4 个新鉴定的 miRNA）及其差异表达的靶 mRNA 被发现参与精子获能相关的 PI3K-Akt、MAPK、cAMP-PKA 和 Ca2+ 信号通路。我们的研究首次提供了公猪精子的完整 miRNA 和转录组图谱。我们的研究结果为了解公猪精子的 RNA 谱以及未来阐明与哺乳动物精子获能相关的潜在分子机制提供了重要的见解。本文的在线版本 (10.1186/s12864-018-5132-9) 包含补充材料，可供授权用户使用。
Capacitation, a prerequisite for oocyte fertilization, is a complex process involving series of structural and functional changes in sperms such as membrane modifications, modulation of enzyme activities, and protein phosphorylation. In order to penetrate and fertilize an oocyte, mammalian sperms must undergo capacitation. Nevertheless, the process of sperm capacitation remains poorly understood and requires further elucidation. In the current study, via high throughput sequencing, we identified and explored the differentially expressed microRNAs (miRNAs) and mRNAs involved in boar sperm capacitation. We identified a total of 5342 mRNAs and 204 miRNAs that were differentially expressed in fresh and capacitated boar sperms. From these, 12 miRNAs (8 known and 4 newly identified miRNAs) and their differentially expressed target mRNAs were found to be involved in sperm capacitation-related PI3K-Akt, MAPK, cAMP-PKA and Ca2+signaling pathways. Our study is first to provide the complete miRNA and transcriptome profiles of boar sperm. Our findings provide important insights for the understanding of the RNA profile in boar sperm and future elucidation of the underlying molecular mechanism relevant to mammalian sperm capacitation. The online version of this article (10.1186/s12864-018-5132-9) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
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