Characteristic rotational behaviors of rod-shaped cargo revealed by automated five-dimensional single particle tracking.
Characteristic rotational behaviors of rod-shaped cargo revealed by automated five-dimensional single particle tracking.
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自动五维单粒子跟踪揭示杆状货物的特征旋转行为
DOI：
10.1038/s41467-017-01001-9
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发表时间：
2017-10-12
影响因子：
16.6
通讯作者：
Fang N
中科院分区：
文献类型：
--
作者：
Chen K;Gu Y;Sun W;Bin Dong;Wang G;Fan X;Xia T;Fang N
我们报告了一种自动单粒子跟踪技术，用于跟踪活细胞中各向异性等离子体金纳米棒探针的 x、y、z 坐标、方位角和仰角。这五个空间坐标统称为5D。该方法克服了微分干涉显微镜中区分旋转运动和平移运动的长期挑战，从而以高精度全面揭示纳米级运动。转铁蛋白包被的内吞金纳米棒货物最初经历主动旋转扩散并在膜上表现出特征性旋转运动。然后，当货物被封闭在网格蛋白涂层的凹坑中时，它们会减慢主动旋转并经历一段安静期，然后在裂变后恢复主动旋转扩散，最终被运离原来的进入点。最后，准确解析货物的 3D 轨迹和伴随的旋转运动，以呈现活细胞中的细胞内运输过程。
We report an automated single particle tracking technique for tracking thex,y,zcoordinates, azimuthal and elevation angles of anisotropic plasmonic gold nanorod probes in live cells. These five spatial coordinates are collectively referred to as 5D. This method overcomes a long-standing challenge in distinguishing rotational motions from translational motions in thez-axis in differential interference contrast microscopy to result in full disclosure of nanoscale motions with high accuracy. Transferrin-coated endocytic gold nanorod cargoes initially undergo active rotational diffusion and display characteristic rotational motions on the membrane. Then as the cargoes being enclosed in clathrin-coated pits, they slow down the active rotation and experience a quiet period before they restore active rotational diffusion after fission and eventually being transported away from the original entry spots. Finally, the 3D trajectories and the accompanying rotational motions of the cargoes are resolved accurately to render the intracellular transport process in live cells.
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影响因子：
64.5
作者：
Kaksonen, M;Toret, CP;Drubin, DG
通讯作者：
Drubin, DG
DOI：
10.1073/pnas.0703094104
发表时间：
2007-10-09
影响因子：
11.1
作者：
Brangwynne, Clifford P.;MacKintosh, F. C.;Weitz, David A.
通讯作者：
Weitz, David A.
DOI：
10.1073/pnas.0803711105
发表时间：
2008-08-19
影响因子：
11.1
作者：
Chen Chen;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
影响因子：
5.7
作者：
Grafmueller, Andrea;Voth, Gregory A.
通讯作者：
Voth, Gregory A.
影响因子：
16.6
作者：
Gu, Yan;Sun, Wei;Fang, Ning
通讯作者：
Fang, Ning