Adaptive immune receptor features related to breast cancer tissue in Kenyan patients: high immunoglobulin gene expression and high levels of gamma-delta T-cells.
Adaptive immune receptor features related to breast cancer tissue in Kenyan patients: high immunoglobulin gene expression and high levels of gamma-delta T-cells.
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DOI：
10.1007/s10549-023-06897-0
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发表时间：
2023-05
影响因子：
3.8
通讯作者：
Makokha, Francis W.
中科院分区：
文献类型：
--
作者：
Mwangi, Kennedy W.;Kamita, Moses K.;Waweru, Jacqueline W.;Sayed, Shahin;Figueroa, Jonine D.;Ambs, Stefan;Cios, Konrad J.;Blanck, George;Makokha, Francis W.
乳腺癌（BC）免疫反应的表征可以为干预点提供信息，例如应用免疫治疗。在这项研究中，我们试图从代表肯尼亚患者的基因组学文件中恢复和表征适应性免疫受体（IR）重组读数，以更好地了解与这些患者特异性相关的免疫应答。我们使用先前应用的算法和软件从代表22名肯尼亚BC患者的癌症和邻近正常组织样品获得生产性IR重组读数。从RNAseq和外显子组文件中，与边缘组织样品相比，从肿瘤样品中回收的T细胞受体（TCR）重组读段显著更多。此外，在肿瘤样品中，免疫球蛋白（IG）基因以比TCR基因高得多的水平表达（p值= 0.0183）。而且，与边缘组织IG CDR 3相比，肿瘤IG CDR 3始终代表更多带正电荷的氨基酸R基团。对于肯尼亚患者，高水平的IG表达，代表特定的CDR3化学，与BC相关。这些结果为支持肯尼亚BC患者特异性免疫干预的研究奠定了基础。
Characterization of the breast cancer (BC) immune response may provide information for a point of intervention, such as application of immunotherapeutic treatments. In this study, we sought to recover and characterize the adaptive immune receptor (IR) recombination reads from genomics files representing Kenyan patients, to better understand the immune response specifically related to those patients. We used a previously applied algorithm and software to obtain productive IR recombination reads from cancer and adjacent normal tissue samples representing 22 Kenyan BC patients. From both the RNAseq and exome files, there were significantly more T-cell receptor (TCR) recombination reads recovered from tumor samples compared to marginal tissue samples. Also, the immunoglobulin (IG) genes were expressed at a much higher level than the TCR genes (p-value = 0.0183) in the tumor samples. And, the tumor IG CDR3s consistently represented more positively charged amino acid R-groups, in comparison to the marginal tissue, IG CDR3s. For Kenyan patients, a high level of IG expression, representing specific CDR3 chemistries, was associated with BC. These results lay the foundation for studies that could support specific immunotherapeutic interventions for Kenyan BC patients.
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影响因子：
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通讯作者：
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