CRISPR-Based COVID-19 Testing: Toward Next-Generation Point-of-Care Diagnostics.
CRISPR-Based COVID-19 Testing: Toward Next-Generation Point-of-Care Diagnostics.
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DOI：
10.3389/fcimb.2021.663949
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发表时间：
2021
影响因子：
5.7
通讯作者：
Liu C
中科院分区：
文献类型：
--
作者：
Ganbaatar U;Liu C
随着COVID-19大流行的持续，人们正以惊人的速度被感染，个人在不知不觉中传播疾病，每天都有更多的人丧生。目前迫切需要一种简单、快速、早期和敏感的COVID-19疾病即时检测方法。然而，目前的测试方法不能满足这种需求。最近，基于成簇的规则间隔短回文重复序列（CRISPR）的检测方法由于其简单性、高灵敏度和特异性而在基于核酸的分子测试中受到了极大的关注。本综述探讨了各种基于CRISPR的COVID-19检测方法和相关诊断设备。与任何新兴技术一样，基于CRISPR/Cas的核酸检测方法在临床和医院的实际应用中必须克服几个挑战。更重要的是，这些检测方法不仅限于COVID-19，还可以应用于检测任何类型的病原体、病毒和真菌，这些病原体、病毒和真菌可能在资源有限的环境中威胁人类、农业和食品工业。基于CRISPR/Cas的检测方法有可能在不久的将来变得更简单，更可靠，更实惠，更快，这对于实现即时诊断非常重要。
As the COVID-19 pandemic continues, people are becoming infected at an alarming rate, individuals are unknowingly spreading disease, and more lives are lost every day. There is an immediate need for a simple, rapid, early and sensitive point-of-care testing for COVID-19 disease. However, current testing approaches do not meet such need. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)-based detection methods have received substantial attention for nucleic acid-based molecular testing due to their simplicity, high sensitivity and specificity. This review explores the various CRISPR-based COVID-19 detection methods and related diagnostic devices. As with any emerging technology, CRISPR/Cas-based nucleic acid testing methods have several challenges that must be overcome for practical applications in clinics and hospitals. More importantly, these detection methods are not limited to COVID-19 but can be applied to detect any type of pathogen, virus, and fungi that may threaten humans, agriculture, and food industries in resource-limited settings. CRISPR/Cas-based detection methods have the potential to become simpler, more reliable, more affordable, and faster in the near future, which is highly important for achieving point-of-care diagnostics.
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DOI：
10.1002/advs.202001300
发表时间：
2020-10
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
--
作者：
Ma P;Meng Q;Sun B;Zhao B;Dang L;Zhong M;Liu S;Xu H;Mei H;Liu J;Chi T;Yang G;Liu M;Huang X;Wang X
通讯作者：
Wang X
DOI：
10.1073/pnas.2010196117
发表时间：
2020-10-13
影响因子：
11.1
作者：
Lee RA;Puig H;Nguyen PQ;Angenent-Mari NM;Donghia NM;McGee JP;Dvorin JD;Klapperich CM;Pollock NR;Collins JJ
通讯作者：
Collins JJ
影响因子：
16.6
作者：
Ding X;Yin K;Li Z;Lalla RV;Ballesteros E;Sfeir MM;Liu C
通讯作者：
Liu C
影响因子：
12.6
作者：
Ding X;Yin K;Li Z;Sfeir MM;Liu C
通讯作者：
Liu C
影响因子：
16.6
作者：
Barnes, Kayla G.;Lachenauer, Anna E.;Sabeti, Pardis C.
通讯作者：
Sabeti, Pardis C.