Gene expression meta-analysis in diffuse low-grade glioma and the corresponding histological subtypes.
Gene expression meta-analysis in diffuse low-grade glioma and the corresponding histological subtypes.
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弥漫性低级别胶质瘤及相应组织学亚型的基因表达荟萃分析
DOI：
10.1038/s41598-017-12087-y
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发表时间：
2017-09-18
影响因子：
4.6
通讯作者：
Han P
中科院分区：
文献类型：
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作者：
Wang S;Jin F;Fan W;Liu F;Zou Y;Hu X;Xu H;Han P
弥漫性低级别胶质瘤（DLGG）是一种分化良好、生长缓慢的肿瘤，具有进展为高级别胶质瘤的内在趋势。遗传改变在DLGG发育中的潜在作用尚未完全阐明。因此，本研究对8个独立的、公开可用的微阵列数据集（包括291个DLGG和83个非胶质瘤（NG）样本）进行了整合基因表达荟萃分析，以确定与DLGG相关的基因表达特征。使用INMEX，与NG相比，在DLGG中鉴定了708个差异表达基因（DEG）（385个上调基因和323个下调基因）。此外，497 DEG（222上调和275下调基因）对应于两种组织学类型进行了鉴定。其中，HIP1R的高表达与总生存率的增加显著相关，而TBXAS 1的高表达与总生存率的降低显著相关。此外，基于网络的Meta分析发现FN 1和APP是DLGG的关键枢纽基因，PTPN 6和CUL3是星形细胞瘤相对于少突胶质细胞瘤的关键枢纽基因。进一步免疫组化证实MTHFD 2和RNP在DLGG中呈阳性表达，而RBP4在NG中呈阳性表达。这些发现揭示了DLGG患者诊断和治疗的潜在分子生物标志物，并为未来的研究提供了丰富和新颖的候选库。
Diffuse low-grade glioma (DLGG) is a well-differentiated, slow-growing tumour with an inherent tendency to progress to high-grade glioma. The potential roles of genetic alterations in DLGG development have not yet been fully delineated. Therefore, the current study performed an integrated gene expression meta-analysis of eight independent, publicly available microarray datasets including 291 DLGGs and 83 non-glioma (NG) samples to identify gene expression signatures associated with DLGG. Using INMEX, 708 differentially expressed genes (DEGs) (385 upregulated and 323 downregulated genes) were identified in DLGG compared to NG. Furthermore, 497 DEGs (222 upregulated and 275 downregulated genes) corresponding to two histological types were identified. Of these, high expression ofHIP1Rsignificantly correlated with increased overall survival, whereas high expression ofTBXAS1significantly correlated with decreased overall survival. Additionally, network-based meta-analysis identifiedFN1andAPPas the key hub genes in DLGG compared with NG.PTPN6andCUL3were the key hub genes identified in the astrocytoma relative to the oligodendroglioma. Further immunohistochemical validation revealed that MTHFD2 and SPARC were positively expressed in DLGG, whereas RBP4 was positively expressed in NG. These findings reveal potential molecular biomarkers for diagnosis and therapy in patients with DLGG and provide a rich and novel candidate reservoir for future studies.
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基于生物信息学的胶质母细胞瘤关键基因预测与分析。
影响因子：
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作者：
Long H;Liang C;Zhang X;Fang L;Wang G;Qi S;Huo H;Song Y
通讯作者：
Song Y
DOI：
10.1056/nejmoa1407279
发表时间：
2015-06-25
期刊：
The New England journal of medicine
影响因子：
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作者：
Eckel-Passow JE;Lachance DH;Molinaro AM;Walsh KM;Decker PA;Sicotte H;Pekmezci M;Rice T;Kosel ML;Smirnov IV;Sarkar G;Caron AA;Kollmeyer TM;Praska CE;Chada AR;Halder C;Hansen HM;McCoy LS;Bracci PM;Marshall R;Zheng S;Reis GF;Pico AR;O'Neill BP;Buckner JC;Giannini C;Huse JT;Perry A;Tihan T;Berger MS;Chang SM;Prados MD;Wiemels J;Wiencke JK;Wrensch MR;Jenkins RB
通讯作者：
Jenkins RB
影响因子：
3.7
作者：
Gao MC;Bellugi U;Dai L;Mills DL;Sobel EM;Lange K;Korenberg JR
通讯作者：
Korenberg JR
影响因子：
11.2
作者：
Hoshida Y;Nijman SM;Kobayashi M;Chan JA;Brunet JP;Chiang DY;Villanueva A;Newell P;Ikeda K;Hashimoto M;Watanabe G;Gabriel S;Friedman SL;Kumada H;Llovet JM;Golub TR
通讯作者：
Golub TR
人类高级星形细胞瘤的基因表达谱。
影响因子：
--
作者：
Liu Z;Yao Z;Li C;Lu Y;Gao C
通讯作者：
Gao C