Identification of CUG-BP1/EDEN-BP target mRNAs in Xenopus tropicalis.
Identification of CUG-BP1/EDEN-BP target mRNAs in Xenopus tropicalis.
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DOI：
10.1093/nar/gkn031
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发表时间：
2008-04
影响因子：
14.9
通讯作者：
Audic Y
中科院分区：
文献类型：
--
作者：
Graindorge A;Le Tonquèze O;Thuret R;Pollet N;Osborne HB;Audic Y
许多动物的早期发育依赖于母体储存的mRNA的转录后调节。特别是，在非洲爪蟾卵母细胞成熟和早期有丝分裂周期中，母体mRNA的翻译受到严格控制。已知胚胎脱腺苷酸化元件（EDEN）及其相关蛋白EDEN-BP触发携带EDEN的若干mRNA的去腺苷酸化和翻译沉默。这种非洲爪蟾RNA结合蛋白是人类蛋白CUG-BP 1/CELF 1的直系同源物。编码细胞周期调节因子和参与notch途径的蛋白质的五种mRNA已被鉴定为被EDEN/EDEN-BP去腺苷化。为了确定新的EDEN-BP目标，我们免疫沉淀EDEN-BP/mRNA复合物从热带爪蟾卵提取物。我们使用微阵列鉴定了153个mRNA作为EDEN-BP的新结合靶点。对新鉴定的EDEN-BP靶标的3′端非翻译区的序列分析显示，推定的EDEN序列富集。EDEN-BP结合的一个子集的目标，证实在体外和体内。在新发现的靶点中，Cdk 1是卵母细胞成熟和细胞周期进程的关键参与者，其3′ UTR特异性靶向受精后EDEN-BP依赖性去腺苷化。
The early development of many animals relies on the posttranscriptional regulations of maternally stored mRNAs. In particular, the translation of maternal mRNAs is tightly controlled during oocyte maturation and early mitotic cycles in Xenopus. The Embryonic Deadenylation ElemeNt (EDEN) and its associated protein EDEN-BP are known to trigger deadenylation and translational silencing to several mRNAs bearing an EDEN. This Xenopus RNA-binding protein is an ortholog of the human protein CUG-BP1/CELF1. Five mRNAs, encoding cell cycle regulators and a protein involved in the notch pathway, have been identified as being deadenylated by EDEN/EDEN-BP. To identify new EDEN-BP targets, we immunoprecipitated EDEN-BP/mRNA complexes from Xenopus tropicalis egg extracts. We identified 153 mRNAs as new binding targets for EDEN-BP using microarrays. Sequence analyses of the 3′ untranslated regions of the newly identified EDEN-BP targets reveal an enrichment in putative EDEN sequences. EDEN-BP binding to a subset of the targets was confirmed both in vitro and in vivo. Among the newly identified targets, Cdk1, a key player of oocyte maturation and cell cycle progression, is specifically targeted by its 3′ UTR for an EDEN-BP-dependent deadenylation after fertilization.
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影响因子：
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