Classical complement activation on human erythrocytes in subjects with systemic lupus erythematosus and a history of autoimmune hemolytic anemia.
Classical complement activation on human erythrocytes in subjects with systemic lupus erythematosus and a history of autoimmune hemolytic anemia.
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全身性红斑狼疮和自身免疫性溶血性贫血病史的受试者中人类红细胞的经典补体激活。
DOI：
10.1177/0961203320936347
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发表时间：
2020-09
期刊：
影响因子：
2.6
通讯作者：
Cunnion K
中科院分区：
文献类型：
--
作者：
Hair P;Goldman DW;Li J;Petri M;Krishna N;Cunnion K
自身免疫性溶血性贫血（AIHA）是系统性红斑狼疮（SLE）的一种严重表现，与显著的发病率和死亡率相关。为了更全面地了解致病途径，我们利用来自患有SLE和活动性AIHA或AIHA病史的受试者的血清来评价经典补体途径、抗红细胞抗体和免疫复合物。为了评价抗体介导的红细胞表面补体活化，如AIHA中发生的那样，将O型血红细胞与来自19名患有SLE和AIHA病史的受试者的血清孵育。循环抗红细胞抗体和免疫复合物用基于ELISA的测定法测量。总体而言，90%的SLE受试者有AIHA病史，但无活动性临床溶血，具有可测量的抗红细胞抗体。在具有抗红细胞抗体的受试者中，53%的受试者表现出红细胞表面上的补体调理作用>阴性对照的两倍，29%的受试者产生过敏毒素C5a。对于有AIHA病史的SLE受试者，循环抗红细胞抗体的持续存在以及由此产生的红细胞补体调理作用和过敏毒素的产生表明这些补体效应物可能导致慢性发病率和AIHA复发风险。
Autoimmune hemolytic anemia (AIHA) is a serious manifestation of systemic lupus erythematosus (SLE) associated with significant morbidity and mortality. In order to more fully understand the causative pathways, we utilized sera from subjects with SLE and active AIHA, or a history of AIHA, to evaluate the classical complement pathway, anti- erythrocyte antibodies, and immune complexes. To evaluate antibody-mediated complement activation on the surface of erythrocytes, as occurs in AIHA, blood type O erythrocytes were incubated with sera from 19 subjects with SLE and a history of AIHA. Circulating anti- erythrocyte antibodies and immune complexes were measured with ELISA-based assays. In total, 90% of subjects with SLE and a history of AIHA, but not active clinical hemolysis, had measurable anti- erythrocyte antibodies. Of those with anti-erythrocyte antibody, 53% demonstrated complement opsonization on the erythrocyte surface >twofold above negative control and 29% generated the anaphylatoxin C5a. For subjects with SLE and a history of AIHA, the persistence of circulating anti-erythrocyte antibodies and resultant erythrocyte complement opsonization and anaphylatoxin generation suggests the possibility that these complement effectors contribute to chronic morbidity and risk of AIHA relapse.
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影响因子：
2.6
作者：
Orbai AM;Truedsson L;Sturfelt G;Nived O;Fang H;Alarcón GS;Gordon C;Merrill J;Fortin PR;Bruce IN;Isenberg DA;Wallace DJ;Ramsey-Goldman R;Bae SC;Hanly JG;Sanchez-Guerrero J;Clarke AE;Aranow CB;Manzi S;Urowitz MB;Gladman DD;Kalunian KC;Costner MI;Werth VP;Zoma A;Bernatsky S;Ruiz-Irastorza G;Khamashta MA;Jacobsen S;Buyon JP;Maddison P;Dooley MA;Van Vollenhoven RF;Ginzler E;Stoll T;Peschken C;Jorizzo JL;Callen JP;Lim SS;Fessler BJ;Inanc M;Kamen DL;Rahman A;Steinsson K;Franks AG Jr;Sigler L;Hameed S;Pham N;Brey R;Weisman MH;McGwin G Jr;Magder LS;Petri M
通讯作者：
Petri M
自身免疫性溶血性贫血的发病机理的新见解。
DOI：
10.1159/000439002
发表时间：
2015-09
期刊：
Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie
影响因子：
--
作者：
Barcellini W
通讯作者：
Barcellini W
影响因子：
3.5
作者：
Lafarge, Antoine;Bertinchamp, R.;Mariotte, E.
通讯作者：
Mariotte, E.
影响因子：
4.8
作者：
Harris, FM;Smith, SK;Bell, JD
通讯作者：
Bell, JD
影响因子：
2.4
作者：
Berentsen, Sigbjorn;Randen, Ulla;Tjonnfjord, Geir E.
通讯作者：
Tjonnfjord, Geir E.