Impact of administration route on nanocarrier biodistribution in a murine colitis model.
Impact of administration route on nanocarrier biodistribution in a murine colitis model.
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DOI：
10.1080/17458080.2022.2134563
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发表时间：
2022
影响因子：
2.8
通讯作者：
Swanson, Kelly S.
中科院分区：
文献类型：
--
作者：
Applegate, Catherine C.;Deng, Hongping;Kleszynski, Brittany L.;Cross, Tzu-Wen L.;Konopka, Christian J.;Dobrucki, L. Wawrzyniec;Nelson, Erik R.;Wallig, Matthew A.;Smith, Andrew M.;Swanson, Kelly S.
炎症性肠病（IBD）的发病率在全球范围内呈上升趋势。尽管目前IBD的诊断和疾病监测测试能敏感地检测肠道炎症，但它们缺乏正电子发射断层扫描（PET）的分子和细胞特异性。在这项概念验证研究中，我们使用放射性标记的巨噬细胞靶向纳米载体探针（64Cu-NOTA-D500），通过口服、灌肠和腹腔给药，来评估右旋糖酐硫酸钠（DSS）诱导的小鼠结肠炎模型中健康和病变组织生物分布的递送途径依赖性。探针在肠组织中摄取的高受试者间变异性被归一化为肝脏或全肠的摄取而降低。健康和DSS结肠炎动物肠道正常摄取的差异在口服和IP途径中最大。肝脏绝对摄取的差异反映了IBD病理的可能的次要诊断指标。这些结果应该为IBD和相关肠道疾病的炎症靶向造影剂的临床前开发提供信息，以提高诊断准确性。
The incidence of inflammatory bowel disease (IBD) is increasing worldwide. Although current diagnostic and disease monitoring tests for IBD sensitively detect gut inflammation, they lack the molecular and cellular specificity of positron emission tomography (PET). In this proof-of-concept study, we use a radiolabeled macrophage-targeted nanocarrier probe (64Cu-NOTA-D500) administered by oral, enema, and intraperitoneal routes to evaluate the delivery route dependence of biodistribution across healthy and diseased tissues in a murine model of dextran sodium sulfate (DSS)-induced colitis. High inter-subject variability of probe uptake in intestinal tissue was reduced by normalization to uptake in liver or total intestines. Differences in normalized uptake between healthy and DSS colitis animal intestines were highest for oral and IP routes. Differences in absolute liver uptake reflected a possible secondary diagnostic metric of IBD pathology. These results should inform the preclinical development of inflammation-targeted contrast agents for IBD and related gut disorders to improve diagnostic accuracy.
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影响因子：
17.1
作者：
Kumar R;Roy I;Ohulchanskky TY;Vathy LA;Bergey EJ;Sajjad M;Prasad PN
通讯作者：
Prasad PN
影响因子：
15.8
作者：
Heazlewood CK;Cook MC;Eri R;Price GR;Tauro SB;Taupin D;Thornton DJ;Png CW;Crockford TL;Cornall RJ;Adams R;Kato M;Nelms KA;Hong NA;Florin TH;Goodnow CC;McGuckin MA
通讯作者：
McGuckin MA
一种口服核苷酸递送载体，靶向结肠巨噬细胞，用于治疗炎症性肠病。
影响因子：
14
作者：
Huang, Zhen;Gan, Jingjing;Dong, Lei
通讯作者：
Dong, Lei
影响因子：
10.8
作者：
HOVGAARD, L;BRONDSTED, H
通讯作者：
BRONDSTED, H
影响因子：
35.7
作者：
Alatab, Sudabeh;Sepanlou, Sadaf G.;Naghavi, Mohsen
通讯作者：
Naghavi, Mohsen