CPHEN-011: Comprehensive phenotyping of murine lung resident lymphocytes after recovery from pneumococcal pneumonia.
CPHEN-011: Comprehensive phenotyping of murine lung resident lymphocytes after recovery from pneumococcal pneumonia.
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CPHEN-011：肺炎球菌肺炎恢复后小鼠肺驻留淋巴细胞的综合表型。
DOI：
10.1002/cyto.a.24522
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发表时间：
2022-11
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影响因子：
--
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从肺炎球菌（Spn）肺炎恢复诱导组织驻留记忆CD4+ TRM细胞、BRM细胞和抗体分泌浆细胞在经验丰富的肺中的发展。这些组织驻留淋巴细胞赋予针对随后的血清型不匹配的Spn的致死性攻击的保护（称为异型免疫）。虽然传统的流式细胞术和门控策略支持使用有限的一组标志物预先鉴定细胞，但发现新的组织驻留淋巴细胞需要稳定的平台，该平台可以处理更大组的表型标志物并使其本身适用于无偏聚类方法。在这份报告中，我们利用全谱流式细胞术（FSFC）的力量，开发一个全面的面板的表型标记，允许识别多个子集的组织驻留淋巴细胞在SPN经验丰富的小鼠肺。使用Phenograph算法在这个多维数据上，我们确定了肺驻留适应性免疫景观中不可预见的异质性，其中包括TRM和BRM细胞的意外子集。此外，使用由我们的无监督聚类数据告知的常规门控策略，我们证实它们在Spn经历的肺中的存在与异型免疫潜在相关，并将CD73定义为TRM细胞上的高表达标志物。因此，我们的研究强调了FSFC在组织驻留适应性免疫相关的确证性和发现性研究中的实用性。
Recovery from pneumococcal (Spn) pneumonia induces development of tissue resident memory CD4+ TRM cells, BRM cells and antibody secreting plasma cells in experienced lungs. These tissue resident lymphocytes confer protection against subsequent lethal challenge by serotype mismatched Spn (termed as heterotypic immunity). While traditional flow cytometry and gating strategies support premeditated identification of cells using a limited set of markers, discovery of novel tissue resident lymphocytes necessitates stable platforms that can handle larger sets of phenotypic markers and lends itself to unbiased clustering approaches. In this report, we leverage the power of full spectrum flow cytometry (FSFC) to develop a comprehensive panel of phenotypic markers that allows identification of multiple subsets of tissue resident lymphocytes in Spn-experienced murine lungs. Using Phenograph algorithm on this multidimensional data, we identify unforeseen heterogeneity in lung resident adaptive immune landscape which includes unexpected subsets of TRM and BRM cells. Further, using conventional gating strategy informed by our unsupervised clustering data, we confirm their presence exquisitely in Spn-experienced lungs as potentially relevant to heterotypic immunity and define CD73 as a highly expressed marker on TRM cells. Thus, our study emphasizes the utility of FSFC for confirmatory and discovery studies relating to tissue resident adaptive immunity.
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DOI：
10.1084/jem.20062571
发表时间：
2007-09-03
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Anderson SM;Tomayko MM;Ahuja A;Haberman AM;Shlomchik MJ
通讯作者：
Shlomchik MJ
影响因子：
15.9
作者：
Barker, Kimberly A.;Etesami, Neelou S.;Mizgerd, Joseph P.
通讯作者：
Mizgerd, Joseph P.
影响因子：
16.6
作者：
Belkina, Anna C.;Ciccolella, Christopher O.;Snyder-Cappione, Jennifer E.
通讯作者：
Snyder-Cappione, Jennifer E.
影响因子：
32.4
作者：
Alugupalli, KR;Leong, JM;Gerstein, RM
通讯作者：
Gerstein, RM
影响因子：
3.7
作者：
Amu, S.;Gjertsson, I.;Brisslert, M.
通讯作者：
Brisslert, M.